A highly selective and easily acquisitive near-infrared fluorescent probe for detection and imaging of hydrogen sulfide in cells

Abstract

Hydrogen sulfide (H₂S) plays a substantial role as a messenger in the physiological and pathological processes of many diseases. Recently, the fluorescence probe of H₂S based on organic dye has attracted great attention. However, the emission of many probes is in the UV-vis region (400-600 nm), so it has the disadvantages of shallow tissue penetration and more vulnerable to spontaneous fluorescence interference. Although several H₂S probes have been developed that emit more than 650 nm, there is a complex structure difficult to synthesize or unstable in storage. Aimed at simply structural and easily synthesized H₂S fluorescent probes with emission wavelength more than 650 nm, a novel near-infrared (NIR) probe (NIR-H₂S) here was rationally designed with 4-(2-carboxyphenyl)-7-(diethylamino)-2-(4-hydroxystyryl)chromenylium (NIR-OH) as a fluorescent dye and 2,4-dinitrophenyl moiety as a recognition group. Addition of H₂S, the "turn-on" NIR fluorescence response at 736 nm of NIR-H₂S was displayed, accompanied by a visual colour change from purple to green when excited at 686 nm. As an easily acquisitive H₂S probe, NIR-H₂S has been successfully applied to cell imaging for H₂S detection with the advantages such as long fluorescence emission, low toxicity, high sensitivity and strong selectivity.

Keywords: Cell imaging; Fluorescent probe; H(2)S; Naked-eye; Near-infrared.