The Intertwined Role of 8-oxodG and G4 in Transcription Regulation

Int J Mol Sci. 2023 Jan 19;24(3):2031. doi: 10.3390/ijms24032031.

Abstract

The guanine base in nucleic acids is, among the other bases, the most susceptible to being converted into 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) when exposed to reactive oxygen species. In double-helix DNA, 8-oxodG can pair with adenine; hence, it may cause a G > T (C > A) mutation; it is frequently referred to as a form of DNA damage and promptly corrected by DNA repair mechanisms. Moreover, 8-oxodG has recently been redefined as an epigenetic factor that impacts transcriptional regulatory elements and other epigenetic modifications. It has been proposed that 8-oxodG exerts epigenetic control through interplay with the G-quadruplex (G4), a non-canonical DNA structure, in transcription regulatory regions. In this review, we focused on the epigenetic roles of 8-oxodG and the G4 and explored their interplay at the genomic level.

Keywords: 8-oxodG; BER; DNA damage; G4; PQS; transcription regulation.

Publication types

  • Review

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • DNA / chemistry
  • DNA Damage*
  • DNA Repair
  • Deoxyguanosine*

Substances

  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine
  • DNA

Grants and funding

This research was supported by “Programma per il Finanziamento della Ricerca di Ateneo Linea A” (FRA) 2022 (University of Naples Federico II) to Stefano Amente; National Center for Gene Therapy and Drugs based on RNA Technology-CN00000041—PNRR, MUR, Italy to Stefano Amente; National Center for Gene Therapy and Drugs based on RNA Technology-CN00000041—PNRR, MUR, Italy to B.M.; AIRC IG 23066 to B.M.