Antibodies to expanded virus antigen panels show elevated diagnostic sensitivities in multiple sclerosis and optic neuritis

Helena Gåsland; Nicole H Trier; Cecilie Kyllesbech; Anette H Draborg; Rimantas Slibinskas; Evaldas Ciplys; Jette L Frederiksen; Gunnar Houen

doi:10.1016/j.imlet.2023.02.003

Antibodies to expanded virus antigen panels show elevated diagnostic sensitivities in multiple sclerosis and optic neuritis

Immunol Lett. 2023 Feb:254:54-64. doi: 10.1016/j.imlet.2023.02.003. Epub 2023 Feb 9.

Authors

Helena Gåsland¹, Nicole H Trier², Cecilie Kyllesbech¹, Anette H Draborg³, Rimantas Slibinskas⁴, Evaldas Ciplys⁴, Jette L Frederiksen², Gunnar Houen⁵

Affiliations

¹ Department of Neurology, Rigshospitalet Glostrup, Valdemar Hansens vej 13, 2600 Glostrup, Denmark; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
² Department of Neurology, Rigshospitalet Glostrup, Valdemar Hansens vej 13, 2600 Glostrup, Denmark.
³ Department of Autoimmunology, Statens Serum Institut, Ørestads boulevard 5, 2300 Copenhagen S, Denmark.
⁴ Institute of Biotechnology, University of Vilnius, Saulėtekio al. 7, 10257 Vilnius, Lithuania.
⁵ Department of Neurology, Rigshospitalet Glostrup, Valdemar Hansens vej 13, 2600 Glostrup, Denmark; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark; Department of Autoimmunology, Statens Serum Institut, Ørestads boulevard 5, 2300 Copenhagen S, Denmark. Electronic address: gunnar.houen@regionh.dk.

PMID: 36764611
DOI: 10.1016/j.imlet.2023.02.003

Abstract

An antigen panel consisting of Epstein-Barr, measles, mumps, varicella zoster and rubella viruses (EMMRZ) was recently presented, which may aid in the diagnosis of multiple sclerosis (MS). The aim of this study was to validate and extend the EMMRZ panel. Various candidates, such as Cytomegalovirus and John Cunningham virus were analysed in relapsing-remitting MS (RRMS) and optic neuritis (ON) samples by enzyme-linked immunosorbent assay. IgG levels were elevated in RRMS samples and correlations were found between serum and cerebrospinal fluid levels. Cohort-dependent optimized panels were obtained for RRMS and ON, which obtained the highest sensitivity when combined with the status of oligoclonal bands.

Keywords: Antibodies; Antibody correlations; Antibody index; Multiple sclerosis; Optic neuritis; Virus antigens.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral
Antigens, Viral
Humans
Immunoglobulin G
Multiple Sclerosis*
Multiple Sclerosis, Relapsing-Remitting*
Optic Neuritis*

Substances

Immunoglobulin G
Antibodies, Viral
Antigens, Viral