Production of a novel laccase from Ceratorhiza hydrophila and assessing its potential in natural dye fixation and cytotoxicity against tumor cells

Yasmin M Elsaba; Heba M El-Hennawi; Mona M Ibrahim; Hala R Wehaidy

doi:10.1186/s43141-023-00473-1

Production of a novel laccase from Ceratorhiza hydrophila and assessing its potential in natural dye fixation and cytotoxicity against tumor cells

J Genet Eng Biotechnol. 2023 Feb 9;21(1):14. doi: 10.1186/s43141-023-00473-1.

Authors

Yasmin M Elsaba¹, Heba M El-Hennawi², Mona M Ibrahim³, Hala R Wehaidy⁴

Affiliations

¹ Botany and Microbiology Department, Faculty of Sciences, Helwan University, Cairo, Egypt.
² Dyeing, Printing and Textile Auxiliaries Department, National Research Centre, Dokki, Giza, Egypt.
³ Plant Biotechnology Department, National Research Centre, Dokki, Giza, Egypt.
⁴ Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, Egypt. halolty2009@yahoo.com.

Abstract

Background: Flavonoid natural dyes have gained attention because they are nontoxic and eco-friendly. However, they do not work effectively with artificial fibers and require the use of mordants, which are considered as hazardous chemicals. Laccase enzyme catalyzes the oxidation of phenols, forming phenoxyl radicals that undergo a further polymerization process. So, laccase can oxidize flavonoid dyes, and it can be used instead of harmful mordants in flavonoid dye fixation on cotton fabrics. Laccases also are involved in a variety of metabolic processes, and they have anti-proliferative effects toward HepG2 and MCF-7 tumor cells.

Results: Among fifteen fungal isolates, the fungus Ceratorhiza hydrophila isolated from the submerged plant Myriophyllum spicatum was selected as the most potent laccase producer. Optimization of the production medium resulted in a 9.9-fold increase in laccase productivity. The partially purified Ceratorhiza hydrophila laccase could successfully improve the affinity of cotton fabrics toward quercetin (flavonoid) dye with excellent color fastness properties. The partially purified laccase also showed anti-proliferative activity against HepG2 and MCF-7 tumor cells. However, high laccase concentration is required to estimate IC50.

Conclusions: Ceratorhiza hydrophila MK387081 is an excellent laccase producer. The partially purified laccase from Ceratorhiza hydrophila can be used in textile dyeing and printing processes as a safer alternative to the conventional hazardous mordants. Also, it can be used in preparation of cancer treatment drugs. However, further studies are needed to investigate IC50 for both cell types at higher laccase concentrations.

Keywords: Ceratorhiza hydrophila MK387081; Flavonoid dye fixation; Laccase cytotoxicity.