The role of xanthine dioxygenase in the biosynthetic pathway of 2-aza-8-oxohypoxanthine of Lepista sordida

Mihaya Kotajima; Jae-Hoon Choi; Tomohiro Suzuki; Jing Wu; Hirofumi Hirai; David C Nelson; Hitoshi Ouchi; Makoto Inai; Hideo Dohra; Hirokazu Kawagishi

doi:10.1093/bbb/zbad005

The role of xanthine dioxygenase in the biosynthetic pathway of 2-aza-8-oxohypoxanthine of Lepista sordida

Biosci Biotechnol Biochem. 2023 Mar 21;87(4):420-425. doi: 10.1093/bbb/zbad005.

Authors

Mihaya Kotajima¹, Jae-Hoon Choi^{1

2

3

4

5}, Tomohiro Suzuki^{5

6}, Jing Wu^{3

5}, Hirofumi Hirai^{1

2

3

4

5}, David C Nelson⁷, Hitoshi Ouchi⁸, Makoto Inai⁸, Hideo Dohra^{2

4

5}, Hirokazu Kawagishi^{3

5}

Affiliations

¹ Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, Japan.
² Graduate School of Integrated Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, Japan.
³ Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, Japan.
⁴ Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, Japan.
⁵ Research Institute for Mushroom Science, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, Japan.
⁶ Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi, Japan.
⁷ Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
⁸ School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, Japan.

PMID: 36756780
DOI: 10.1093/bbb/zbad005

Abstract

2-Azahypoxanthine (AHX) and 2-aza-8-oxohypoxanthine (AOH), discovered as causal substances of fairy rings are known to be endogenous in the fairy ring-forming Lepista sordida. In this study, we showed that xanthine dioxygenase, an a-ketoglutarate-dependent dioxygenase, might catalyze the conversion of AHX to AOH in the fungus. Furthermore, this enzyme is the first reported molybdopterin-independent protein of hypoxanthine metabolism.

Keywords: Lepista sordida; AHX; AOH; XDO.

MeSH terms

Agaricales* / metabolism
Biosynthetic Pathways
Dioxygenases* / metabolism
Hypoxanthines / metabolism
Xanthine / metabolism

The role of xanthine dioxygenase in the biosynthetic pathway of 2-aza-8-oxohypoxanthine of Lepista sordida

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