Plasma riboflavin fluorescence as a diagnostic marker of mesenteric ischemia-reperfusion injury in rats

Xueqin Wu; Lun-Zhang Guo; Yi-Hung Liu; Yu-Cheng Liu; Po-Lun Yang; Yun-Shiuan Leung; Hwan-Ching Tai; Tzung-Dau Wang; Jesse Chih-Wei Lin; Chao-Lun Lai; Yueh-Hsun Chuang; Chih-Hsueh Lin; Pi-Tai Chou; I-Rue Lai; Tzu-Ming Liu

doi:10.1016/j.thromres.2023.01.032

Plasma riboflavin fluorescence as a diagnostic marker of mesenteric ischemia-reperfusion injury in rats

Thromb Res. 2023 Mar:223:146-154. doi: 10.1016/j.thromres.2023.01.032. Epub 2023 Feb 3.

Authors

Xueqin Wu¹, Lun-Zhang Guo², Yi-Hung Liu³, Yu-Cheng Liu¹, Po-Lun Yang², Yun-Shiuan Leung⁴, Hwan-Ching Tai⁵, Tzung-Dau Wang⁶, Jesse Chih-Wei Lin⁷, Chao-Lun Lai⁸, Yueh-Hsun Chuang⁹, Chih-Hsueh Lin¹⁰, Pi-Tai Chou¹¹, I-Rue Lai¹², Tzu-Ming Liu¹³

Affiliations

¹ Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau SAR, China; MOE Frontiers Science Center for Precision Oncology, University of Macau, Macau SAR, China.
² Department of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
³ Graduate Institute of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan.
⁴ Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
⁵ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Department of Laboratory Medicine, School of Public Health, Xiamen University, Xiamen 361102, P. R. China. Electronic address: hctai@xmu.edu.cn.
⁶ Cardiovascular Center and Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital and College of Medicine, Taipei 10002, Taiwan.
⁷ Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan.
⁸ Department of Internal Medicine, National Taiwan University Hospital Hsin-Chu Branch, Hsin-Chu, Taiwan; Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan; Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan. Electronic address: chaolunlai@ntu.edu.tw.
⁹ Department of Anesthesiology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan. Electronic address: cyh771048@ntu.edu.tw.
¹⁰ Department of Nutrition, College of Medical and Health Care, Hungkuang University, Taichung City 433304, Taiwan.
¹¹ Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan. Electronic address: chop@ntu.edu.tw.
¹² Graduate Institute of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan; Department of Surgery, National Taiwan University Hospital, Taipei 100229, Taiwan. Electronic address: iruelai@gmail.com.
¹³ Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau SAR, China; MOE Frontiers Science Center for Precision Oncology, University of Macau, Macau SAR, China. Electronic address: tmliu@um.edu.mo.

PMID: 36753876
DOI: 10.1016/j.thromres.2023.01.032

Abstract

Due to the delayed and vague symptoms, it is difficult to early diagnose mesenteric ischemia injuries in the dynamics of acute illness, leading to a 60-80 % mortality rate. Here, we found plasma fluorescence spectra can rapidly assess the severity of mesenteric ischemia injury in animal models. Ischemia-reperfusion damage of the intestine leads to multiple times increase in NADH, flavins, and porphyrin auto-fluorescence of blood. The fluorescence intensity ratio between blue-fluorophores and flavins can reflect the occurrence of shock. Using liquid chromatography and mass spectroscopy, we confirm that riboflavin is primarily responsible for the increased flavin fluorescence. Since humans absorb riboflavin from the intestine, its increase in plasma may indicate intestinal mucosa injury. Our work suggests a self-calibrated and reagent-free approach to identifying the emergence of fatal mesenteric ischemia in emergency departments or intensive care units.

Keywords: Mesenteric ischemia; Plasma fluorophores; Ultrahigh performance liquid chromatography.

MeSH terms

Animals
Disease Models, Animal
Humans
Mesenteric Ischemia*
Rats
Rats, Wistar
Reperfusion Injury*
Riboflavin

Substances

Riboflavin