An optical monitoring device combining a smartphone with a polychromatic ratiometric fluorescence-colorimetric paper sensor was developed to detect Hg2+ and S2- in water and seafood. This monitoring included the detection of food deterioration and was made possible by processing the sensing data with a machine learning algorithm. The polychromatic fluorescence sensor was composed of blue fluorescent carbon quantum dots (CDs) (BU-CDs) and green and red fluorescent CdZnTe quantum dots (QDs) (named GN-QDs and RD-QDs, respectively). The experimental results and density functional theory (DFT) prove that the incorporation of Zn can improve the stability and quantum yield of CdZnTe QDs. According to the dynamic and static quenching mechanisms, GN-QDs and RD-QDs were quenched by Hg2+ and sulfide, respectively, but BU-CDs were not sensitive to them. The system colors change from green to red to blue as the concentration of the two detectors rises, and the limits of detection (LOD) were 0.002 and 1.488 μM, respectively. Meanwhile, the probe was combined with the hydrogel to construct a visual sensing intelligent test strip, which realized the monitoring of food freshness. In addition, a smartphone device assisted by multiple machine learning methods was used to text Hg2+ and sulfide in real samples. It can be concluded that the fabulous stability, sensitivity, and practicality exhibited by this sensing mechanism give it unlimited potential for assessing the contents of toxic and hazardous substances Hg2+ and sulfide.
Keywords: Hg2+; machine learning; polychromatic fluorescence; smartphone; sulfide.