A long-wavelength mitochondria-targeted fluorescent probe for imaging of peroxynitrite during dexamethasone treatment

Jun Tang; Ziyi Li; Chuchu Qiang; Yan Han; Lifang Yang; Li Zhu; Tan Dang; Gairong Chen; Yong Ye

doi:10.1016/j.saa.2023.122429

A long-wavelength mitochondria-targeted fluorescent probe for imaging of peroxynitrite during dexamethasone treatment

Spectrochim Acta A Mol Biomol Spectrosc. 2023 May 5:292:122429. doi: 10.1016/j.saa.2023.122429. Epub 2023 Feb 3.

Authors

Jun Tang¹, Ziyi Li², Chuchu Qiang², Yan Han², Lifang Yang², Li Zhu², Tan Dang², Gairong Chen², Yong Ye³

Affiliations

¹ School of Chemistry and Materials Engineering, Xinxiang University, Xinxiang, Henan 453003, China. Electronic address: 847537061@qq.com.
² School of Chemistry and Materials Engineering, Xinxiang University, Xinxiang, Henan 453003, China.
³ Green Catalysis Center, and College of Chemistry, Zhengzhou University, Zhengzhou 450001, China.

PMID: 36750010
DOI: 10.1016/j.saa.2023.122429

Abstract

Peroxynitrite (ONOO^-), as a strong oxidizing reactive nitrogen substance (RNS), is generated endogenously by cells. Its visualization research is crucial to understand relevant disease processes. Herein, we reported a long-wavelength mitochondria-targeted fluorescence "turn on" probe TL. The probe TL could react with ONOO^- by using 4-(Bromomethyl)benzeneboronic as a reactive site, which exhibited outstanding characteristics for detection of ONOO^-, thus improving response time (about 50 s), sensitivity (DL, 10.1 nM), and emission wavelength (667 nm). Besides, TL displayed well mitochondria targeting and biological visualizing of exogenous and endogenous ONOO^- in biological systems. Finally, TL was used to monitor high concentration of dexamethasone-induced an up-regulation of ONOO^-. This indicated that TL has excellent potential to study the fluctuation of ONOO^- in the physiological and pathological system.

Keywords: Dexamethasone; Fluorescence probe; Mitochondria-targeted; ONOO(−).

MeSH terms

Dexamethasone / analysis
Fluorescent Dyes* / chemistry
Microscopy, Fluorescence / methods
Mitochondria / chemistry
Optical Imaging
Peroxynitrous Acid* / analysis

Substances

Fluorescent Dyes
Peroxynitrous Acid
Dexamethasone