Halomonas getboli sp. nov., a halotolerant bacteria isolated from a salt flat

Abstract

A novel Gram-stain-negative, rod-shaped, cream-coloured, motile, halotolerant bacterium, designated as YJPS3-2^T, was isolated from saltern sediment of the Yellow sea in Yongyu-do, Republic of Korea. Strain YJPS3-2^T grew at pH 5.0-10.0 (optimum, pH 7.0), 4-40 °C (optimum, 30 °C) and with 1-15% (w/v) NaCl (optimum 3 %). The 16S rRNA gene sequence analysis indicated that strain YJPS3-2^T was closely related to those of Halomonas halophila F5-7^T (98.75 %), Halomonas salina F8-11^T (98.74 %), Halomonas smyrnensis AAD6^T (98.66 %), Halomonas organivorans G-16.1^T (98.34 %), Halomonas koreensis SS20^T (97.98 %) and Halomonas beimenensis NTU-107^T (96.93 %). The average nucleotide identity and digital DNA-DNA hybridization values between YJPS3-2^T and related type strains were 86.9-91.6 % and 32.0-44.8 %. Strain YJPS3-2^T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10 %) were C_{16 : 0} (31.4 %), C_{19 : 0} ω8c cyclo (16.3 %), C_{17 : 0} cyclo (11.9 %) and C_{12 : 0} 3-OH (10.4 %). The polar lipids consisted of phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain YJPS3-2^T is 68.1mol %. Based on the polyphasic taxonomic evidence presented in this study, YJPS3-2^T should be classified as representing a novel species within the genus Halmonas, for which name Halomonas getboli is proposed, with the type strain YJPS3-2^T (= KCTC 92124^T=KACC 22561^T=JCM 35085^T).

Keywords: Getbol; Halomonas; halophile; halotolerant bacteria; marine bacteria.