Double membrane platelet-rich fibrin (PRF) - Synovium succeeds in regenerating cartilage defect at the knee: An experimental study on rabbit

Ahmad Taufik S; Bayu Tirta Dirja; Dwikora Novembri Utomo; Muhammad Andry Usman; Muhammad Sakti; Muhammad Ruksal Saleh; Mochammad Hatta; Budu

doi:10.1016/j.heliyon.2023.e13139

Double membrane platelet-rich fibrin (PRF) - Synovium succeeds in regenerating cartilage defect at the knee: An experimental study on rabbit

Heliyon. 2023 Jan 22;9(2):e13139. doi: 10.1016/j.heliyon.2023.e13139. eCollection 2023 Feb.

Authors

Ahmad Taufik S^{1

2}, Bayu Tirta Dirja¹, Dwikora Novembri Utomo³, Muhammad Andry Usman⁴, Muhammad Sakti⁴, Muhammad Ruksal Saleh⁴, Mochammad Hatta², Budu⁵

Affiliations

¹ Faculty of Medicine Mataram University, Mataram, Indonesia.
² Department of Molecular Biology and Immunology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
³ Department of Orthopaedic, Faculty of Medicine Airlangga University, Surabaya, Indonesia.
⁴ Department of Orthopaedic, Faculty of Medicine Hasanuddin University, Makasar, Indonesia.
⁵ Department of Opthalmology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.

Abstract

Background: This study aims to prove the healing results (regeneration) in cartilage defects using a combination treatment of microfractures and transplantation synovium-platelet rich fibrin (S-PRF).

Methods: A cartilage defect was made in the trochlear groove of the knee of adult New Zealand white rabbits, and was classified into three treatment groups. The group 1 was cartilage defect without treatment, 2 with microfracture treatment, and 3 with microfracture covered with a synovium-platelet rich fibrin (S-PRF) membrane. Twelve weeks after the intervention, the animals were macroscopically and histologically examined, and evaluated by the International Cartilage Repair Society (ICRS). Additionally, the expression of aggrecan and type 2 collagen was examined by real-time-PCR.

Results: The ICSR scores for macroscopic were significantly higher in the microfracture and S-PRF transplant group than in the other groups. Also, the ICSR scores for histology were significantly higher in this group. The expression of aggrecan and type 2 collagen was higher in the group that received complete treatment.

Conclusions: Microfractures and transplantation of synovium-platelet rich fibrin (S-PRF) can regenerate knee cartilage defects which have been shown to increase the expression of mRNA aggrecan and mRNA type 2 collagen resulting in excellent repair.

Keywords: Cartilage defect; Microfracture; Platelet-rich fibrin; Synovium.