iPSC-derived organ-on-a-chip models for personalized human genetics and pharmacogenomics studies

Victoria E J M Palasantzas; Isabel Tamargo-Rubio; Kieu Le; Jelle Slager; Cisca Wijmenga; Iris H Jonkers; Vinod Kumar; Jingyuan Fu; Sebo Withoff

doi:10.1016/j.tig.2023.01.002

iPSC-derived organ-on-a-chip models for personalized human genetics and pharmacogenomics studies

Trends Genet. 2023 Apr;39(4):268-284. doi: 10.1016/j.tig.2023.01.002. Epub 2023 Feb 5.

Authors

Victoria E J M Palasantzas¹, Isabel Tamargo-Rubio², Kieu Le², Jelle Slager², Cisca Wijmenga², Iris H Jonkers², Vinod Kumar³, Jingyuan Fu¹, Sebo Withoff⁴

Affiliations

¹ Department of Genetics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands; Department of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
² Department of Genetics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
³ Department of Genetics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands; Department of Internal Medicine and Radboud Centre for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.
⁴ Department of Genetics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Electronic address: s.withoff@umcg.nl.

PMID: 36746737
DOI: 10.1016/j.tig.2023.01.002

Abstract

Genome-wide association studies (GWAS) have now correlated hundreds of genetic variants with complex genetic diseases and drug efficacy. Functional characterization of these factors remains challenging, particularly because of the lack of human model systems. Molecular and nanotechnological advances, in particular the ability to generate patient-specific PSC lines, differentiate them into diverse cell types, and seed and combine them on microfluidic chips, have led to the establishment of organ-on-a-chip (OoC) platforms that recapitulate organ biology. OoC technology thus provides unique personalized platforms for studying the effects of host genetics and environmental factors on organ physiology. In this review we describe the technology and provide examples of how OoCs may be used for disease modeling and pharmacogenetic research.

Keywords: iPSCs; intestine-on-a-chip; liver-on-a-chip; organ-on-a-chip; personalized medicine; pharmacogenomics; vessel-on-a-chip.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Genome-Wide Association Study
Human Genetics
Humans
Induced Pluripotent Stem Cells*
Microphysiological Systems
Pharmacogenetics