Simultaneous determination of zearalenone and alternariol mycotoxins in oil samples using mixed molecularly imprinted polymer beads

Tamara Moya-Cavas; Fernando Navarro-Villoslada; Javier Lucas Urraca; Luis Antonio Serrano; Guillermo Orellana; María Cruz Moreno-Bondi

doi:10.1016/j.foodchem.2023.135538

Simultaneous determination of zearalenone and alternariol mycotoxins in oil samples using mixed molecularly imprinted polymer beads

Food Chem. 2023 Jun 30:412:135538. doi: 10.1016/j.foodchem.2023.135538. Epub 2023 Jan 21.

Authors

Tamara Moya-Cavas¹, Fernando Navarro-Villoslada², Javier Lucas Urraca³, Luis Antonio Serrano⁴, Guillermo Orellana⁴, María Cruz Moreno-Bondi¹

Affiliations

¹ Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias, 2, 28040 Madrid, Spain.
² Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias, 2, 28040 Madrid, Spain. Electronic address: fenavi@ucm.es.
³ Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias, 2, 28040 Madrid, Spain. Electronic address: jurracar@quim.ucm.es.
⁴ Department of Organic Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias, 2, 28040 Madrid, Spain.

PMID: 36738530
DOI: 10.1016/j.foodchem.2023.135538

Abstract

This work reports the optimization of a method using Molecularly Imprinted Polymers (MIPs) for the simultaneous determination of zearalenone and alternariol mycotoxins. The method was optimized using a chemometric approach where in the optimized conditions, the cartridges with a mixture (50:50, w/w) of both MIPs, were loaded with 30 mL of sample, washed with 2 mL of ACN/water (20/80, v/v) and eluted with 2.5 mL of trifluoroacetic acid/MeOH (3/97, v/v). The extracts were analyzed by HPLC coupled to a fluorescence detector (FLD). The optimized method has been applied and validated to the analysis of the mycotoxins in maize, sunflower and olive oils samples with a limit of detection of 5 and 2 µg kg^-1, respectively. Recoveries were in the range of 94 % to 108 % (RSD < 6 %) for zearalenone and 92 % to 113 % (RSD < 5 %) for alternariol. The results were confirmed by HPLC-MS/MS.

Keywords: Alternariol; Molecularly imprinted polymer; Mycotoxin; Solid Phase Extraction; Zearalenone.

MeSH terms

Chromatography, High Pressure Liquid / methods
Molecular Imprinting* / methods
Molecularly Imprinted Polymers
Mycotoxins* / analysis
Solid Phase Extraction / methods
Tandem Mass Spectrometry
Zearalenone* / analysis

Substances

Zearalenone
Mycotoxins
alternariol
Molecularly Imprinted Polymers