Screen printed electrodes on interfacial Pt-CuO/carbon nanofiber functional ink for real-time qualification of cell released hydrogen peroxide

Ning Zhang; Mengqi Tong; Zhuanzhuan Shi; Jianyu Yang; Bo Chen; Changming Li; Chunxian Guo

doi:10.1016/j.aca.2023.340829

Screen printed electrodes on interfacial Pt-CuO/carbon nanofiber functional ink for real-time qualification of cell released hydrogen peroxide

Anal Chim Acta. 2023 Mar 8:1245:340829. doi: 10.1016/j.aca.2023.340829. Epub 2023 Jan 12.

Authors

Ning Zhang¹, Mengqi Tong², Zhuanzhuan Shi², Jianyu Yang², Bo Chen², Changming Li³, Chunxian Guo⁴

Affiliations

¹ School of Materials Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China; Institute of Advanced Cross-field Science, College of Life Science, Qingdao University, Qingdao, 266071, PR China.
² School of Materials Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China.
³ School of Materials Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China; Institute of Clean Energy & Advanced Materials, Southwest University, Chongqing, 400715, China; Institute of Advanced Cross-field Science, College of Life Science, Qingdao University, Qingdao, 266071, PR China. Electronic address: ecmli@swu.edu.cn.
⁴ School of Materials Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China. Electronic address: cxguo@usts.edu.cn.

PMID: 36737132
DOI: 10.1016/j.aca.2023.340829

Abstract

Screen printed electrode (SPE) on carbon-based inks exhibits promising applications in biosensing, environment protection and food safety. We report here a unique carbon-based material comprising Pt-CuO nanocrystal interfacially anchored on functionalized carbon nanofiber (Pt-CuO@FCNF) and its functional ink to build SPE for ultrasensitive detection of cell released H₂O₂. Pt-CuO@FCNF is fabricated using a one-pot and mass production method through direct pyrolysis of Pt and CuO precursors together with FCNF. FCNF with 1-D structure and high electrical conductivity can interfically anchor Pt-CuO nanocrystal, which synergically promotes rich active site and catalytic activity towards H₂O₂. Pt-CuO@FCNF exhibits a wide linear response of 0.4 μM-11 mM, a low detection limit of 17 nM, a fast response time of 1.0 s, and good selectivity. Eventually, Pt-CuO@FCNF SPE realizes real-time and ultrasensitive qualification of H₂O₂ released from both normal and cancer cells.

Keywords: Carbon nanofiber; Functional ink; Interface engineering; Live-cell assay; Nanozyme; Screen printed electrode.

MeSH terms

Biosensing Techniques* / methods
Carbon / chemistry
Electrodes
Hydrogen Peroxide
Ink
Nanofibers* / chemistry

Substances

Carbon
cupric oxide
Hydrogen Peroxide