Cadmium treatment induces endoplasmic reticulum stress and unfolded protein response in Arabidopsisthaliana

Abstract

We report about the response of Arabidopsis thaliana to chronic and temporary Cd²⁺ stress, and the Cd²⁺ induced activation of ER stress and unfolded protein response (UPR). Cd²⁺-induced UPR proceeds mainly through the bZIP60 arm, which in turn activates relevant ER stress marker genes such as BiP3, CNX, PDI5 and ERdj3B in a concentration- (chronic stress) or time- (temporary stress) dependent manner. A more severe Cd-stress triggers programmed cell death (PCD) through the activation of the NAC089 transcription factor. Toxic effects of Cd²⁺ exposure are reduced in the Atbzip28/bzip60 double mutant in terms of primary root length and fresh shoot weight, likely due to reduced UPR and PCD activation. We also hypothesised that the enhanced Cd²⁺ tolerance of the Atbzip28/bzip60 double mutant is due to an increase in brassinosteroids signaling, since the amount of the brassinosteroid insensitive1 receptor (BRI1) protein decreases under Cd²⁺ stress only in Wt plants. These data highlight the complexity of the UPR pathway, since the ER stress response is strictly related to the type of the treatment applied and the multifaceted connections of ER signaling. The reduced sensing of Cd²⁺ stress in plants with UPR defects can be used as a novel strategy for phytoremediation.

Keywords: Cadmium; Endoplasmic reticulum; Endoplasmic reticulum associated degradation; Unfolded protein response; bZIP28; bZIP60.