Melatonin is a growth regulator that improves the growth and chlorophyll (chl) content in plants. This study aims to investigate the effect of melatonin pretreatment on chl synthesis and fluorescence parameters in Malva parviflora exposed to cadmium (Cd). The 42-day-old plants were transferred to nutrient solutions containing 50 μM melatonin. After two days, some plants were exposed to 50 μM Cd. Eight days after Cd treatment, some indicators related to chl fluorescence and some biochemical parameters were measured. In this study, melatonin increased chl content and chl a/pheophytin a (pheo a) ratio, chlorophyllide a (chlide a), porphyrin compounds, and 5-aminolevulinic acid (5-ALA) in the presence of Cd. However, it decreased chl a/chlide a ratio under these conditions. Whereas Cd treatment resulted in significant reductions in photochemical activity and electron transfer rate in PSII, melatonin improved photochemical efficiency of PSII by reducing the toxic effect of Cd on the activity of the oxygen evolving complex (OEC) on the electron donor site and reducing non-photochemical quenching (NPQ). Based on the results, it appears that melatonin can maintain the chl content of plants exposed to Cd by increasing the precursors of the chl biosynthesis pathway and reducing its degradation rate. These results may, at least in our experimental conditions, partly explain the reason for the improved yield and growth of Cd-exposed plants when pretreated with melatonin.
Keywords: Cadmium; Chlorophyll content; Chlorophyll fluorescence; Melatonin.
© Prof. H.S. Srivastava Foundation for Science and Society 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.