Marker assisted stacking of Ty3, Mi1.2 and Ph3 resistance alleles for leaf curl, root knot and late blight diseases in tomato

Deepak Maurya; Arnab Mukherjee; Bhagyashree; Surabhi Sangam; Randhir Kumar; Shirin Akhtar; Tirthartha Chattopadhyay

doi:10.1007/s12298-022-01277-2

Marker assisted stacking of Ty3, Mi1.2 and Ph3 resistance alleles for leaf curl, root knot and late blight diseases in tomato

Physiol Mol Biol Plants. 2023 Jan;29(1):121-129. doi: 10.1007/s12298-022-01277-2. Epub 2023 Jan 5.

Authors

Deepak Maurya¹, Arnab Mukherjee², Bhagyashree², Surabhi Sangam¹, Randhir Kumar¹, Shirin Akhtar¹, Tirthartha Chattopadhyay²

Affiliations

¹ Department of Horticulture (Vegetable and Floriculture), Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar 813210 India.
² Department of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar 813210 India.

Abstract

Developing multiple disease resistance through naturally available host resistance alleles is a challenging as well as rewarding area of research. Availability of host resistance alleles and the reliability of their identification through diagnostic molecular markers have paved the way for stacking of these resistance alleles for developing important genetic resources in tomato. Here we report the marker assisted stacking of Ty3, Mi1.2 and Ph3 alleles, governing leaf curl, root knot and late blight disease resistance, respectively, in superior F₄ segregants of tomato derived from two diverse parents (i.e., BRDT-1 and H-88-78-1). Marker assisted selection was applied only on morphologically superior segregants at F₂ and F₃ generations, which helped us in identifying suitable lines even from a relatively small population. The diagnostic values of the employed molecular markers advocate that the identified superior segregants, carrying all the three aforementioned resistance alleles in homozygous condition, are suitable to be explored as valuable genetic resources for developing multiple disease resistance through rapid introgression of these genes in different genetic background of tomato. Identification of suitable segregants derived from these lines should be promising for obtaining improved cultivars in near future. Nevertheless, these lines might be further explored to decipher the intrinsic details of host's resistance mechanism involving genetic interactions between different resistance factors.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-022-01277-2.

Keywords: Early generation fixation; Host resistance alleles; Introgression; Marker assisted breeding; Molecular markers; Multiple disease resistance.

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