Tannic acid-derived selective capture of bacteria from apple juice

Abstract

Given the enormous burden pathogens pose on human health, rapid capture and removal of bacteria for sterilization or further bacterial detection is essential. Herein, tannic acid-functionalized virus-like Fe₃O₄ (vFe₃O₄-TA) was established for bacterial enrichment. We investigated the ability of vFe₃O₄-TA to capture Gram-negative bacteria (E. coli, S. flex and S. typhi) and Gram-positive bacteria (S. aureus, MRSA and LM), respectively. Compared to the capture efficiency of <15 % for Gram-negative bacteria, vFe₃O₄-TA showed excellent selectivity and efficiency in isolating Gram-positive bacteria with >87 % removal efficiency. GFN-xTB semiempirical quantum chemical calculations revealed that the selective recognition originates from the high affinity between TA and peptidoglycan. Without impacting ingredients, the TA-mediated trapper also shows excellent ability to distinguish Gram-positive bacteria in juice samples. These results are expected to reveal the interaction of TA with bacteria, and inaugurate a potential natural safe tool for food safety control, medical treatment and environmental remediation.

Keywords: Adsorption; Gram-positive bacteria; Independent gradient model; Magnetic separation; Tannic acid.