Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway

Xiaoyang Wang; Jie Zhao; Xiaochuan Wang; Jingjuan Zhang; Yi Wang; Xinyue Wang; Shanshan Jia; Nian Shi; Meiqi Lu; Hongxia Su; Jixun Zhang; Duyin Jiang

doi:10.3389/fsurg.2022.1027067

Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway

Front Surg. 2023 Jan 16:9:1027067. doi: 10.3389/fsurg.2022.1027067. eCollection 2022.

Authors

Xiaoyang Wang¹, Jie Zhao², Xiaochuan Wang¹, Jingjuan Zhang¹, Yi Wang¹, Xinyue Wang¹, Shanshan Jia¹, Nian Shi¹, Meiqi Lu¹, Hongxia Su³, Jixun Zhang¹, Duyin Jiang^{1

2}

Affiliations

¹ Department of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, China.
² Emergency Medicine Center, The Second Hospital of Shandong University, Jinan, China.
³ Shandong Nameide Biotechnology Limited Company, Jinan, China.

Abstract

Objective: The bacterial cellulose membrane (BCM) has been widely studied and applied as a new biomaterial for wound healing, but causes pain with frequent dressing changes. Local application of bone marrow mesenchymal stem cells (BMSCs) requires a niche. Furthermore, the effect and mechanism of the BCM combined with BMSCs have not been reported.

Methods: Morphological and chemical identifications of BCMs were investigated by porosity analyses, scanning electron microscopy, and Fourier-transform infrared spectroscopy. Biological wound dressings (BWDs) were prepared by the BCM in combination with BMSCs. The biological effects of BWDs on human dermal fibroblast (HDF) and VEGF-A in human vascular endothelial cells (HuVECs) were detected in vitro, and the effect of BWDs on acute wounds in mice was detected in vivo. Collagen and angiogenesis were evaluated through hematoxylin-eosin staining and Masson staining. The expressions of COL-1 and VEGF-A and the activation of the Notch signaling pathway in vivo and in vitro were detected by quantitative reverse-transcriptase polymerase chain reaction.

Results: The BCM had a nanoscale structure and provided a partial niche for the survival and proliferation of BMSCs. BWDs were successfully prepared and regulated the biological behaviors of wound healing-related cells in vitro and upregulated the expressions of COL-1 in HDF and VEGF-A in HuVECs. BWDs promoted wound healing by increasing collagen type I synthesis and angiogenesis in acute wounds in mice.

Conclusions: BWDs prepared by the combination of nanomaterial BCMs and BMSCs facilitated acute wound healing, which may be regulated by activating the Notch signaling pathway.

Keywords: BMSCs; bacterial cellulose; bioactive wound dressing; nanomaterial; notch signaling pathway; tissue repair.

Grants and funding

This work was supported by the National Natural Science Foundation of China (No: 81372074, 81071560, 81873934), Wang Zhengguo Foundation for Traumatic Medicine (growth factor rejuvenation project, SZYZ-TR-09), Science and Technology Development Projects of Shandong Province (No: 2015GSF118041), the Youth Fund from Natural Science Foundation of Shandong Province (ZR2020QH168).