Selective laser welding in liquid: A strategy for preparation of high-antibacterial activity nanozyme against Staphylococcus aureus

Abstract

Nanozyme was considered as one of the most promising substitutes for antibiotics, due to the selective catalysis for pathogens. In this work, a high-antibacterial activity SOD-like nanozyme based on hybrid Ag/CeO₂ nanocomposite was facilely prepared by using an innovative approach of selective laser welding in liquid. This prepared nanozyme displayed a high antimicrobial effect against Staphylococcus aureus under visible light illumination, the sterilization rate as high as 82.4%, which was 2.93 and 2.99 times higher than those of pure Ag and pure CeO₂, respectively. The enhanced antibacterial activity was attributed to the anchoring of Ag nanospheres on the surface of CeO₂ nanosheets, which induced the reduction of CeO₂ bandgap and boosted the visible light harvesting. Therefore, the charge carriers can be effectively stimulated to produce abundant reactive oxygen species on the Ag/CeO₂ nanocomposite via a SOD-like route. This work demonstrated a facile strategy for the preparation of high-antibacterial activity nanozyme, giving it great potential for scalable application in the biomedical and pharmaceutical industry.

Keywords: Antibacterial activity; Nanozyme; Selective laser welding in liquid; Visible light illumination.