Long Non-Coding RNAs and microRNAs Groups in the Regulation of Expression Level of a Number of Tumor-Associated Genes in Ovarian Cancer

I V Pronina; E A Filippova; O I Brovkina; A M Burdennyy; T P Kazubskaya; D N Kushlinskii; K I Zhordania; A V Karpukhin; V I Loginov; E A Braga; N E Kushlinskii

doi:10.1007/s10517-023-05707-3

Long Non-Coding RNAs and microRNAs Groups in the Regulation of Expression Level of a Number of Tumor-Associated Genes in Ovarian Cancer

Bull Exp Biol Med. 2023 Jan;174(3):354-359. doi: 10.1007/s10517-023-05707-3. Epub 2023 Feb 1.

Authors

Affiliations

¹ Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
² Research Institute of General Pathology and Pathophysiology, Moscow, Russia. burdennyy@gmail.com.
³ N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.
⁴ N. P. Bochkov Research Centre for Medical Genetics, Moscow, Russia.

PMID: 36723744
DOI: 10.1007/s10517-023-05707-3

Abstract

The search for interacting long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs of protein-coding genes through the mechanism of competing endogenous RNAs in tumors of ovarian cancer patients was carried out. The levels of expression of 24 lncRNAs, 20 miRNAs, and 28 mRNAs of protein-coding genes involved in oncogenesis were determined by real-time PCR on a set of representative samples. Correlations between lncRNAs/miRNA and miRNA/mRNA levels in ovarian cancer samples were analyzed. We identified 8 pairs of lncRNAs/miRNA and 17 pairs of miRNA/mRNA, the expression levels of which have a negative correlation. Five triplets of potentially interacting lncRNAs/miRNA/mRNA have been identified, among which the most significant triplet is the OIP5-AS1/miR-203a-3p/ZEB1. The data obtained determine new epigenetic profiles, as well as new potential biomarkers and targets for targeted therapy of ovarian cancer patients.

Keywords: Long non-coding RNAs; Mechanism of competing endogenous RNAs; Ovarian cancer; microRNAs.

MeSH terms

Female
Gene Expression Regulation, Neoplastic / genetics
Gene Regulatory Networks
Humans
MicroRNAs* / genetics
MicroRNAs* / metabolism
Ovarian Neoplasms* / genetics
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism

Substances

MicroRNAs
RNA, Long Noncoding
RNA, Messenger