Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC

Sameer Singh; Anoosha Banerjee; Arnaud Vanden Broeck; Sebastian Klinge

doi:10.1038/s41598-023-28732-8

Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC

Sci Rep. 2023 Jan 31;13(1):1719. doi: 10.1038/s41598-023-28732-8.

Authors

Sameer Singh^{1

2}, Anoosha Banerjee¹, Arnaud Vanden Broeck¹, Sebastian Klinge³

Affiliations

¹ Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA.
² Institut für Medizinische Physik und Biophysik, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
³ Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA. klinge@rockefeller.edu.

Abstract

One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
Cell Membrane
Clone Cells
Epitopes
Gene Editing*
Humans

Substances

Epitopes