A recently developed immunoenzymatic technique, the Dot Immunobinding assay (DIB), has been applied to the rapid detection of staphylococcal enterotoxin B in filtered culture supernatants. The test has been shown to be highly sensitive and specific, easy to perform and rapid (only 4 hours). The use of nitrocellulose paper as the solid phase allows the exact standardization of the amount of antigen employed and it avoids the overnight incubation usually necessary with the conventional enzyme-linked immunosorbent assay (ELISA).