The effectiveness of Ovaprim, Ovopel, and their combinations in artificial reproduction of common rudd Scardinius erythrophthalmus under controlled conditions

Erfan Akbari Nargesi; Bahram Falahatkar; Daniel Żarski; Danial Gorouhi

doi:10.1016/j.theriogenology.2023.01.014

The effectiveness of Ovaprim, Ovopel, and their combinations in artificial reproduction of common rudd Scardinius erythrophthalmus under controlled conditions

Theriogenology. 2023 Mar 15:199:114-120. doi: 10.1016/j.theriogenology.2023.01.014. Epub 2023 Jan 17.

Authors

Erfan Akbari Nargesi¹, Bahram Falahatkar², Daniel Żarski³, Danial Gorouhi⁴

Affiliations

¹ Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran.
² Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran; Department of Marine Sciences, The Caspian Sea Research Basin Center, University of Guilan, Rasht, Guilan, Iran. Electronic address: falahatkar@guilan.ac.ir.
³ Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
⁴ Shahid Ansari Teleost Fish Restocking and Genetic Conservation Center, Rasht, Guilan, Iran.

PMID: 36716592
DOI: 10.1016/j.theriogenology.2023.01.014

Abstract

In this study, a practical protocol for artificial reproduction was developed for the rudd, Scardinius erythrophthalmus, to help to produce this fish for aquaculture industries and also for restocking programs. For this purpose, the efficiency of different hormonal agents and their combinations for spawning induction of female breeders of rudd was evaluated. Ovulations were stimulated using different treatments as: two injections of Ovaprim (Ova), two injections of Ovopel (Ovo), a priming dose of Ovopel with a resolving dose of Ovaprim (Comb1), a priming dose of Ovaprim with a resolving dose of Ovopel (Comb2), and two injections of sterile 0.9% NaCl solution as a control group. During the study, ovulation success, egg developmental competence as well as the performance of the freshly hatched larvae were recorded. There was no ovulation in females from the control group. In hormone-treated groups, 84-100% of fish ovulated, but no differences were observed in ovulation success (P > 0.05). The shortest latency period in treated fish was recorded in the Ovo group (431.6 degree-hour, P < 0.05). The best results in terms of working fecundity (number of obtained eggs per fish), relative fecundity, fertilization success, hatching rate, and embryo survival up to the eyed stage were achieved in the Ova and Comb1 groups (P < 0.05). Moreover, better survival rates up to the absorbing yolk sac stage and the lowest abnormality of larvae were observed in Ova and Comb1 groups (P < 0.05). There was no significant difference in the incubation time among different groups (P > 0.05). The obtained results indicate that two injections of Ovaprim and/or a priming dose of Ovopel with a resolving dose of Ovaprim are suitable for the artificial reproduction of rudd female breeders. Due to the proper effects and ease of use, two injections of Ovaprim could be recommended for the production of this species for restocking or aquaculture programs.

Keywords: Artificial spawning; Broodstock management; Cyprinidae; Hormonal agents; Propagation.

MeSH terms

Animals
Cyprinidae*
Domperidone / pharmacology
Female
Gonadotropin-Releasing Hormone / pharmacology
Reproduction*

Substances

ovaprim
Gonadotropin-Releasing Hormone
Domperidone