Chromatin is composed of DNA and its associated proteins, and has an essential role in all cellular processes, including those taking place during Drosophila oogenesis. In order to understand the molecular basis of chromatin-based processes, such as transcription, it is essential to be able to study how and when different proteins, such as transcription factors, histones and RNA polymerases, interact with chromatin. One of the most popular methods to study this is chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq). Here, we describe a ChIP-seq protocol that has been optimized for Drosophila ovaries, focusing on sample preparation through preliminary data processing.
Keywords: ChIP-seq; Chromatin; Chromatin immunoprecipitation; Drosophila oogenesis; Next-generation sequencing (NGS); Ovaries.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.