A poly (glycerol-sebacate-acrylate) nanosphere enhanced injectable hydrogel for wound treatment

Jiajia Luo; Fenglei Sun; Pinhua Rao; Tonghe Zhu; Yonghang Liu; Juan Du; Sihao Chen; Xiangyun Jin; Jiale Jin; Yi Chai

doi:10.3389/fbioe.2022.1091122

A poly (glycerol-sebacate-acrylate) nanosphere enhanced injectable hydrogel for wound treatment

Front Bioeng Biotechnol. 2023 Jan 12:10:1091122. doi: 10.3389/fbioe.2022.1091122. eCollection 2022.

Authors

Jiajia Luo¹, Fenglei Sun², Pinhua Rao¹, Tonghe Zhu¹, Yonghang Liu¹, Juan Du¹, Sihao Chen¹, Xiangyun Jin³, Jiale Jin⁴, Yi Chai⁵

Affiliations

¹ School of Chemistry and Chemical Engineering, Institute for Frontier Medical Technology, Shanghai Engineering Research Center of Pharmaceutical Intelligent Equipment, Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Non-coding RNA, Shanghai University of Engineering Science, Shanghai, China.
² Department of Neurosurgery, Weifang People's Hospital, Weifang, Shandong, China.
³ Department of Orthopaedics, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
⁴ Spine Lab, Department of Orthopaedic Surgery, The First Affiliated Hospital, Zhejiang University, Hangzhou, China.
⁵ Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Abstract

Wound repair remains a huge clinical challenge, which can cause bleeding, infection, and patient death. In our current research, a bioactive, injectable, multifunctional composite hydrogel doped with nanospheres was prepared with antibacterial and angiogenesis-promoting functions for the treatment of wounds. Amino groups in ε-polylysine (ε-EPL) undergo dynamic Schiff base reaction cross-linking with oxidized hyaluronic acid (OHA), and F127 exhibits unique temperature sensitivity to form an injectable thermosensitive hydrogel (FHE10), which can form a hydrogel to cover the wound at body temperature. Nanospheres (PNs) prepared using poly (glyceryl-sebacate-acrylate) (PGSA) were loaded into hydrogels (FHE10) for promoting wound repair. The prepared FHE10 exhibited rapid gelation, good injectable abilities, and showed resistance to the flourish of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). In vitro investigations showed that FHE10 had good hemocompatibility and cytocompatibility. FHE10@PNs exhibited good proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs) and human foreskin fibroblasts (HFF-1). Furthermore, FHE10@PNs significantly promoted reepithelialization and collagen deposition as well as micro-vascularization compared with the use of FHE10 or PNs alone, thereby accelerating the repair of wounds. In general, this study demonstrated that the multifunctional injectable composite hydrogel showed great potential in wound treatment.

Keywords: antibacterial; injectable hydrogel; poly (glycerol-sebacate-acrylate) nanosphere; vascularization; wound treatment.

Grants and funding

This research was funded by the Shanghai Rising-Star Program (20QC1401300), National Natural Science Foundation of China (81902186), Technology Support Project of Science and Technology Commission of Shanghai Municipality (22S31904700), Construction Project of Shanghai Engineering Technology Research Center (20DZ2255900), and the Shanghai Sailing Program (22YF1423500).