Influence of Nano-Methionine supplementation in drinking water on growth performance, lipid metabolism, and related gene expression in broiler chicken

Set A El-Shobokshy; Magda I Abo-Samaha; Eman M Abd El-Maksoud; Shymaa A Khatab; Asmaa F Khafaga; Gemechu Wirtu

doi:10.5455/javar.2022.i644

Influence of Nano-Methionine supplementation in drinking water on growth performance, lipid metabolism, and related gene expression in broiler chicken

J Adv Vet Anim Res. 2022 Dec 31;9(4):743-753. doi: 10.5455/javar.2022.i644. eCollection 2022 Dec.

Authors

Set A El-Shobokshy¹, Magda I Abo-Samaha², Eman M Abd El-Maksoud³, Shymaa A Khatab⁴, Asmaa F Khafaga⁵, Gemechu Wirtu⁶

Affiliations

¹ Department of Nutrition and Veterinary Clinical Nutrition, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
² Poultry Breeding and Production, Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
³ Biochemistry Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
⁴ Genetics and Genetic Engineering, Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
⁵ Pathology Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
⁶ Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL, USA.

Abstract

Objectives: The study aimed to determine how Nano-Methionine (Nano-Meth) affected growth, lipid metabolism, and relative gene expression for acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), growth hormone receptor (GHR), insulin-like growth factor receptor-1 (IGFR-1), myostatin (MSTN), and cholecystokinin (CCK) genes in broiler chickens.

Materials and methods: A total of 100 1-day-old broilers were randomly assigned into 2 groups: 1) the control group received drinking water without any supplements, and 2) the Nano-Meth group received 10 ml/l of 5% Nano-Meth starting from 1 day old until 35 days old (the end of the experiment).

Results: Nano-Meth improved final body weight, weight gains, feed intake, and feed conversion ratio. Compared to the control group, Nano-Meth significantly lowered the serum levels of triglyceride, cholesterol, very low-density lipoprotein, and low-density lipoprotein in chickens. Nano-Meth significantly increased the serum levels of total protein, albumin, high-density lipoprotein, and glucose more than the control group. Nano-Meth lowered the mRNA gene expression of ACC, FAS, MSTN, and CCK but increased that of GHR and IGFR-1.

Conclusions: We concluded that supplementation with Nano-Meth enhances growth performance and decreases lipid accumulation in broiler chickens.

Keywords: Broiler; Nano-Methionine; gene expression; growth performance; histopathology; lipid.