Zerumbone Protects Rats from Collagen-Induced Arthritis by Inhibiting Oxidative Outbursts and Inflammatory Cytokine Levels

Rana M Alsaffar; Aarif Ali; Shahzada Mudasir Rashid; Sheikh Bilal Ahmad; Faisal K Alkholifi; Majid Shafi Kawoosa; Sheikh Parvaiz Ahmad; Muneeb U Rehman

doi:10.1021/acsomega.2c05749

Zerumbone Protects Rats from Collagen-Induced Arthritis by Inhibiting Oxidative Outbursts and Inflammatory Cytokine Levels

ACS Omega. 2023 Jan 10;8(3):2982-2991. doi: 10.1021/acsomega.2c05749. eCollection 2023 Jan 24.

Authors

Rana M Alsaffar¹, Aarif Ali², Shahzada Mudasir Rashid², Sheikh Bilal Ahmad², Faisal K Alkholifi¹, Majid Shafi Kawoosa³, Sheikh Parvaiz Ahmad⁴, Muneeb U Rehman⁵

Affiliations

¹ Department of Pharmacology & Toxicology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia.
² Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, SKUAST-Kashmir, Shuhama, Alusteng, Srinagar, Jammu and Kashmir 190006, India.
³ Mycoplasma Laboratory, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Alusteng, Srinagar, Jammu and Kashmir 190006, India.
⁴ Department of Statistics, University of Kashmir, Srinagar, Jammu and Kashmir 190006, India.
⁵ Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

Abstract

Rheumatoid arthritis (RA) is an immunocompromised disorder characterized by a marked increase in the synthesis of inflammatory molecules that stimulates the destruction of bones and cartilage. The conventional treatment modalities for RA are associated with adverse side effects and lack sensitivity, suggesting an immediate demand for alternate beneficial therapeutic remedies. The current study sought to understand more about zerumbone's anti-inflammatory properties in diagnosing collagen-induced arthritis (CIA) in experimental animals. The current study observed that zerumbone reduced clinical severity in CIA-induced animals compared to healthy animals. Zerumbone administration significantly decreased (p < 0.001) the concentration of SOD, CAT, GR, and GSH in treatment groups. Zerumbone administration drove down significantly (p < 0.001) the concentration of inflammatory cytokine molecules. Zerumbone was effective in bringing significant changes in levels of MPO, NO, LDH, MMP-8, and ELA. The therapeutic potential of zerumbone was found to be associated with reduced joint destruction and restored normal histology in the cartilage and tissue. Adsorption, distribution, metabolism, excretion, and toxicity studies were used to determine the druglike properties of zerumbone. ProTox-II studies revealed that zerumbone did not possess toxic properties like hepatotoxicity, immunotoxicity, carcinogenicity, mutagenicity, and cytotoxicity. Therefore, the present study evaluated the therapeutic properties of zerumbone in CIA animal models.