PRKX down-regulates TAK1/IRF7 signaling in the antiviral innate immunity of black carp Mylopharyngodon piceus

Xiao Yang; Yue Ai; Liang Chen; Chanyuan Wang; Ji Liu; Jie Zhang; Jun Li; Hui Wu; Jun Xiao; Mingxian Chang; Hao Feng

doi:10.3389/fimmu.2022.999219

PRKX down-regulates TAK1/IRF7 signaling in the antiviral innate immunity of black carp Mylopharyngodon piceus

Front Immunol. 2023 Jan 11:13:999219. doi: 10.3389/fimmu.2022.999219. eCollection 2022.

Authors

Xiao Yang¹, Yue Ai¹, Liang Chen¹, Chanyuan Wang¹, Ji Liu¹, Jie Zhang², Jun Li³, Hui Wu¹, Jun Xiao¹, Mingxian Chang², Hao Feng¹

Affiliations

¹ State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
² State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
³ Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources, College of Biological and Food Engineering, Huaihua University, Huaihua, China.

Abstract

TGF-β-activated kinase-1 (TAK1), tightly related to innate immunity, is phosphorylated and activated by X-linked protein kinase (PRKX) in humans and mammals, which belongs to the c-AMP-dependent protein kinase family. However, the relationship between PRKX and TAK1 remains unknown in teleost. It has been reported in vertebrates for the first time that TAK1 of black carp (bcTAK1) interacts with bcIRF7 and is capable to up-regulate bcIRF7-mediated IFN signaling in our previous study. In this study, the role of PRKX homologue of black carp (Mylopharyngodon piceus) (bcPRKX) in bcTAK1/IFN signaling has been explored. Overexpression of bcPRKX suppressed the transcription of interferon promoters but enhanced the transcription of NF-κB promoter. Mylopharyngodon piceus kidney (MPK) cells transfected with shRNA targeting bcPRKX gene presented enhanced antiviral activity against spring viremia of carp virus (SVCV), in which the mRNA levels of the antiviral proteins were increased, including MX1, Viperin and PKR. Overexpressed bcPRKX dampened bcTAK1/bcIRF7/IFN signaling in the luciferase reporter assay and plaque assay. The interaction between bcTAK1 and bcPRKX has been identified by the immunofluorescence (IF) staining and co-immunoprecipitation (co-IP) assay. In addition, we found that bcPRKX can trigger the degradation of bcTAK1. However, the lysosome inhibitor chloroquine, but not the proteasome inhibitor MG-132, prevented the bcTAK1 degradation mediated by bcPRKX. Thus, we conclude that bcPRKX inhibits bcTAK1/bcIRF7/IFN signaling during the innate immune activation by targeting bcTAK1 and triggers lysosome-dependent degradation of bcTAK1.

Keywords: IRF7; PRKX; SVCV; TAK1; black carp; interferon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carps* / immunology
Immunity, Innate / genetics
Protein Kinases
Rhabdoviridae Infections* / veterinary

Substances

Protein Kinases

Grants and funding

This work was supported by the National Natural Science Foundation of China (U21A20268, 31920103016, 32002415, 32173010, 32002430, 32002383), Hunan Provincial Science and Technology Department (2021NK2025, 2022JJ30383), Hunan Provincial Education Department (20A317), the Training Program for Excellent Young Innovators of Changsha (kq2106031), the State Key Laboratory of Developmental Biology of Freshwater Fish (2020KF008), the Modern Agricultural Industry Program of Hunan Province, and the Fish Disease and Vaccine Research and Development Platform for Postgraduates in Hunan Province.