Spatiotemporal based response for methylene blue removal using surface modified calcium carbonate microspheres coated with Bacillus sp

Noha M Deghiedy; Hanan S El-Bastawisy; Ola M Gomaa

doi:10.1039/d2ra05466c

Spatiotemporal based response for methylene blue removal using surface modified calcium carbonate microspheres coated with Bacillus sp

RSC Adv. 2023 Jan 10;13(3):1842-1852. doi: 10.1039/d2ra05466c. eCollection 2023 Jan 6.

Authors

Noha M Deghiedy¹, Hanan S El-Bastawisy², Ola M Gomaa³

Affiliations

¹ Radiation Polymer Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA) Cairo Egypt.
² Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA) Cairo Egypt.
³ Radiation Microbiology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA) Cairo Egypt ola_gomaa@hotmail.com.

Abstract

Calcium carbonate microspheres are attractive for their biocompatibility, high loading capacity and easy preparation. They can be used in biomedicine and catalytic applications. In the present work, calcium carbonate microspheres were surface modified with polyvinylpyrrolidone (PVP) followed by irradiation at 5 kGy prior to coating with Bacillus sp. cells. To provide cell protection and internal energy storage, polyhydroxybutyrate (PHB) was induced using 3 factors 2 levels factorial design where the order of effect on PHB% was pH > incubation time > glucose concentration. The highest production was 81.68 PHB% at pH 9, 20 g L^-1 glucose and 4 days incubation time. Bacillus sp. cells grown under PHB optimal conditions were used to coat the surface modified calcium carbonate microspheres. Characterization was performed using X-ray diffraction, Fourier Transform Infrared Spectroscopy, Dynamic light Scattering, Zeta potential and Scanning Electron Microscopy. The results obtained confirm the formation and coating of microspheres of 2.34 μm and -16 mV. The prepared microspheres were used in bioremoval of methylene blue dye, the results showed spatiotemporal response for MB-microsphere interaction, where PHB induced Bacillus sp. coated microspheres initially adsorb MB to its outer surface within 1 h but decolorization takes place when the incubation time extends to 18 h. The microspheres can be reused up to 3 times with the same efficiency and with no desorption. These results suggest that the surface modified calcium carbonate can be tailored according to the requirement which can be delivery of biomaterial, bioadsorption or bioremediation.