Effect of particle size on the tissue adhesion and particle floatation of a colloidal wound dressing for endoscopic treatments

Shima Ito; Akihiro Nishiguchi; Tetsushi Taguchi

doi:10.1016/j.actbio.2023.01.041

Effect of particle size on the tissue adhesion and particle floatation of a colloidal wound dressing for endoscopic treatments

Acta Biomater. 2023 Mar 15:159:83-94. doi: 10.1016/j.actbio.2023.01.041. Epub 2023 Jan 25.

Authors

Shima Ito¹, Akihiro Nishiguchi², Tetsushi Taguchi³

Affiliations

¹ Graduate School of Science and Technology, Degree Programs in Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan; Polymers and Biomaterials Field, Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
² Polymers and Biomaterials Field, Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
³ Graduate School of Science and Technology, Degree Programs in Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan; Polymers and Biomaterials Field, Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. Electronic address: TAGUCHI.Tetsushi@nims.go.jp.

PMID: 36706853
DOI: 10.1016/j.actbio.2023.01.041

Abstract

Endoscopic submucosal dissection (ESD) is a minimally invasive technique that is widely used to remove gastrointestinal tumors. However, because the walls of the duodenum and large intestine are thin, perforation can easily occur after ESD. We have previously reported that alkyl group-modified Alaska pollock gelatin-based microparticles (C10Ps) formed a colloidal gel that could adhere to defects and close perforations, driven by hydrophobic interactions. The present study focused on the effect of particle size on the colloidal gel properties and the floatation of C10Ps in the air in the delivery of C10Ps. We prepared C10Ps with different particle sizes from 0.1 to 100 µm. The storage modulus and adhesion strength of the C10P colloidal gel increased with decreasing particle size. All the C10Ps formed a colloidal gel layer on duodenum tissue after being sprayed from an endoscopic device. The underwater stability and burst strength of C10Ps with a particle size of 0.1 and 1 µm were higher than for larger C10Ps. Floating of the small-sized C10Ps in the air was observed. The results indicated that C10Ps with a size of 1 µm had suitable properties for use in endoscopic treatments. STATEMENT OF SIGNIFICANCE: We previously reported tissue adhesive microparticles as a spray-deliverable wound dressing in gastrointestinal tissues. However, their functions depending on particle size have not yet been clarified. In the present study, we prepared decyl group-modified Alaska pollock gelatin nano and microparticles (C10Ps) with different particle sizes from 0.1 to 100 µm and evaluated the effect of particle size on the colloidal gel properties (rheological property, underwater stability and perforation-closing ability) and the floatation of C10Ps in the air in the delivery of C10Ps.

Keywords: Endoscope submucosal dissection; Hydrophobic interaction; Particle size; Perforation closure; Underwater adhesion; Wound dressing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bandages
Gastrointestinal Tract*
Gelatin* / chemistry
Humans
Particle Size
Tissue Adhesions
Treatment Outcome

Substances

Gelatin