Integrative analysis of transcriptome and lipidome reveals fructose pro-steatosis mechanism in goose fatty liver

Rongxue Wei; Chunchun Han; Shouhai Wei; Yongqiang Teng; Liang Li; Hehe Liu; Shengqiang Hu; Bo Kang; Hengyong Xu

doi:10.3389/fnut.2022.1052600

Integrative analysis of transcriptome and lipidome reveals fructose pro-steatosis mechanism in goose fatty liver

Front Nutr. 2023 Jan 10:9:1052600. doi: 10.3389/fnut.2022.1052600. eCollection 2022.

Authors

Rongxue Wei^{1

2}, Chunchun Han^{1

2}, Shouhai Wei^{1

2}, Yongqiang Teng^{1

2}, Liang Li^{1

2}, Hehe Liu^{1

2}, Shengqiang Hu^{1

2}, Bo Kang^{1

2}, Hengyong Xu^{1

2}

Affiliations

¹ Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
² Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.

Abstract

To further explore the fructose pro-steatosis mechanism, we performed an integrative analysis of liver transcriptome and lipidome as well as peripheral adipose tissues transcriptome analysis using samples collected from geese overfed with maize flour (control group) and geese overfed with maize flour supplemented with 10% fructose (treatment group). Overfeeding period of the treatment group was significantly shorter than that of the control group (p < 0.05). Dietary supplementation with 10% fructose induced more severe steatosis in goose liver. Compared with the control group, the treatment group had lower in ceramide levels (p < 0.05). The key differentially expressed genes (DEGs) (control group vs. treatment group) involved in liver fatty acid biosynthesis and steroid biosynthesis were downregulated. The conjoint analysis between DEGs and different lipids showed that fatty acid biosynthesis and steroid biosynthesis were the highest impact score pathways. In conclusion, fructose expedites goose liver lipid accumulation maximization during overfeeding.

Keywords: fructose; goose fatty liver; lipidome; peripheral adipose tissues; transcriptome.

Grants and funding

This experiment was supported by the Natural Science Funds of Sichuan Province (No. 2022NSFSC0059) and the National Natural Science Funds of China (No. 31672413).