Imaging of spinal cord microvasculature holds great potential in directing critical care management of spinal cord injury (SCI). Traditionally, contrast agents are preferred for imaging of the spinal cord vasculature, which is disadvantageous for long-term monitoring of injury. Here, we present FlowMorph, an algorithm that uses mathematical morphology techniques to segment non-contrast Doppler-based videos of rat spinal cord. Using the segmentation, it measures single-vessel parameters such as flow velocity, rate, and radius, with visible cardiac cycles in individual vessels showcasing the spatiotemporal resolution. The segmentation outlines vessels well with little extraneous labeling, and outlines are smooth through time. Radius measurements of perforating vessels are similar to what is seen in the literature through other methods. Verification of the algorithm through comparison to manual measurement and in vitro microphantom standards highlights points of future improvement. This method will be vital for future work studying the vascular effects of SCI and can be adopted to other species as well.
Keywords: Ultrasound; blood flow; mathematical morphology; non-contrast; spinal cord.