Multi-omics analysis of the effects of dietary changes and probiotics on diet-induced obesity

Shiman Jiang; Yuanshuai Su; Qiangqiang Wang; Longxian Lv; Chen Xue; Lvwan Xu; Lanjuan Li

doi:10.1016/j.crfs.2023.100435

Multi-omics analysis of the effects of dietary changes and probiotics on diet-induced obesity

Curr Res Food Sci. 2023 Jan 6:6:100435. doi: 10.1016/j.crfs.2023.100435. eCollection 2023.

Authors

Shiman Jiang¹, Yuanshuai Su¹, Qiangqiang Wang¹, Longxian Lv¹, Chen Xue¹, Lvwan Xu¹, Lanjuan Li^{1

2}

Affiliations

¹ State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou City, 310003, China.
² Jinan Microecological Biomedicine Shandong Laboratory, Jinan, 250021, China.

Abstract

The consumption of a healthy diet is critical for maintaining and promoting human health. In the context of the rapid transformation from a high-fat diet (HFD) to a Mediterranean diet (MD) leading to major systemic changes, we explored the necessity of a transitional standard diet (TSD) between these two varied diets and the adjuvant effect of probiotics. HFD-fed mice were used for studying the changes and benefits of a dietary intervention and probiotic treatment. By measuring multiple systemic alterations such as weight (group B vs. group E, P < 0.05), liver function (AST, group C vs. group E, P < 0.001), and histopathology, we found that an MD, TSD and Bifidobacterium longum all contribute to alleviating lipid deposition and liver injury. The downregulation of IL-17 (group B vs. group E, P < 0.01) and MIP-1α (group B vs. group E, P < 0.001) also demonstrated the anti-inflammatory effects of the TSD. Moreover, we performed multi-omics analysis combined with the 16S sequencing, transcriptome and metabolome results and found that the TSD increased the abundance of the Lactobacillus genus (group C vs. group E, P < 0.01) and effectively lowered lipid accumulation and systemic inflammation. Furthermore, B. longum played an important role in the synergistic effect. The results showed that a TSD might be useful for HFD-induced obesity before drastic dietary changes, and probiotics were also beneficial.

Keywords: Dietary change; Lactobacillus genus; Probiotic; Transitional standard diet.