The Influence of Outer Membrane Protein on Ampicillin Resistance of Vibrio parahaemolyticus

Xiangyu Meng; Danyang Huang; Qing Zhou; Fan Ji; Xin Tan; Jianli Wang; Xiaoyuan Wang

doi:10.1155/2023/8079091

The Influence of Outer Membrane Protein on Ampicillin Resistance of Vibrio parahaemolyticus

Can J Infect Dis Med Microbiol. 2023 Jan 13:2023:8079091. doi: 10.1155/2023/8079091. eCollection 2023.

Authors

Xiangyu Meng¹, Danyang Huang², Qing Zhou¹, Fan Ji¹, Xin Tan², Jianli Wang³, Xiaoyuan Wang^{1

2

3}

Affiliations

¹ School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
² School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Abstract

The antibiotic resistance of the food-borne pathogen Vibrio parahaemolyticus has attracted researchers' attention in recent years, but its molecular mechanism remains poorly understood. In this study, 7 genes encoding outer membrane proteins (OMPs) were individually deleted in V. parahaemolyticus ATCC33846, and the resistance of these 7 mutants to 14 antibiotics was investigated. The results revealed that the resistance of the 7 mutants to ampicillin was significantly increased. Further exploration of 20-gene transcription changes by real time-qPCR (RT-qPCR) demonstrated that the higher ampicillin resistance might be attributed to the expression of β-lactamase and reduced peptidoglycan (PG) synthesis activity through reduced transcription of penicillin-binding proteins (PBPs), increased transcription of l,d-transpeptidases, downregulated d,d-carboxypeptidase, and alanine deficiency. This study provides a new perspective on ampicillin resistance in OMP mutants with respect to PG synthesis.