Metabolome and microbiome analysis revealed the effect mechanism of different feeding modes on the meat quality of Black Tibetan sheep

Xue Zhang; Lijuan Han; Linsheng Gui; Sayed Haidar Abbas Raza; Shengzhen Hou; Baochun Yang; Zhiyou Wang; Ying Ma; Raafat T M Makhlof; Zamzam Alhuwaymil; Samah F Ibrahim

doi:10.3389/fmicb.2022.1076675

Metabolome and microbiome analysis revealed the effect mechanism of different feeding modes on the meat quality of Black Tibetan sheep

Front Microbiol. 2023 Jan 6:13:1076675. doi: 10.3389/fmicb.2022.1076675. eCollection 2022.

Authors

Xue Zhang¹, Lijuan Han¹, Linsheng Gui¹, Sayed Haidar Abbas Raza^{2

3}, Shengzhen Hou¹, Baochun Yang¹, Zhiyou Wang¹, Ying Ma¹, Raafat T M Makhlof^{4

5}, Zamzam Alhuwaymil⁶, Samah F Ibrahim⁷

Affiliations

¹ Department of Animal Science, College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, China.
² College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
³ Safety of Livestock and Poultry Products, College of Food Science, South China Agricultural University, Guangzhou, China.
⁴ Department of Parasitology, Faculty of Medicine, Umm Al Qura University, Mecca, Saudi Arabia.
⁵ Department of Parasitology, Faculty of Medicine, Minia University, Minya, Egypt.
⁶ Organic Department, College of Science and Humanities at Al-Quway'iyah, Shaqra University, Shaqra, Saudi Arabia.
⁷ Department of Clinical Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.

Abstract

Introduction: Black Tibetan sheep is one of the primitive sheep breeds in China that is famous for its great eating quality and nutrient value but with little attention to the relationship between feeding regimes and rumen metabolome along with its impact on the muscle metabolism and meat quality.

Methods: This study applies metabolomics-based analyses of muscles and 16S rDNA-based sequencing of rumen fluid to examine how feeding regimes influence the composition of rumen microbiota, muscle metabolism and ultimately the quality of meat from Black Tibetan sheep. Twenty-seven rams were randomly assigned to either indoor feeding conditions (SG, n = 9), pasture grazing with indoor feeding conditions (BG, n = 9) or pasture grazing conditions (CG, n = 9) for 120 days.

Results: The results showed that, compared with BG and CG, SG improved the quality of Black Tibetan sheep mutton by preventing a decline in pH and increasing fat deposition to enhance the color, tenderness and water holding capacity (WHC) of the Longissimus lumborum (LL). Metabolomics and correlation analyses further indicated that the feeding regimes primarily altered amino acid, lipid and carbohydrate metabolism in muscles, thereby influencing the amino acid (AA) and fatty acid (FA) levels as well as the color, tenderness and WHC of the LL. Furthermore, SG increased the abundance of Christensenellaceae R-7 group, [Eubacterium] coprostanoligenes group, Methanobrevibacter, Ruminococcus 2 and Quinella, decreased the abundance of Lactobacillus, Prevotella 1 and Rikenellaceae RC9 gut group, and showed a tendency to decrease the abundance of Succinivibrio and Selenomonas 1. Interestingly, all of these microorganisms participated in the deposition of AAs and FAs and modified the levels of different metabolites involved in the regulation of meat quality (maltotriose, pyruvate, L-ascorbic acid, chenodeoxycholate, D-glucose 6-phosphate, glutathione, etc.).

Discussion: Overall, the results suggest that feeding Black Tibetan sheep indoors with composite forage diet was beneficial to improve the mouthfeel of meat, its color and its nutritional value by altering the abundance of rumen bacteria which influenced muscle metabolism.

Keywords: 16S rDNA; Black Tibetan sheep; feeding regimes; meat quality; metabolomics.