Data on 2D culture characterisation of potential markers in human HER2-positive breast cancer cell lines

Son H Pham; Lyn R Griffiths; Rachel K Okolicsanyi; Larisa M Haupt

doi:10.1016/j.dib.2022.108880

Data on 2D culture characterisation of potential markers in human HER2-positive breast cancer cell lines

Data Brief. 2023 Jan 7:46:108880. doi: 10.1016/j.dib.2022.108880. eCollection 2023 Feb.

Authors

Son H Pham¹, Lyn R Griffiths¹, Rachel K Okolicsanyi¹, Larisa M Haupt^{1

2}

Affiliations

¹ Stem Cell and Neurogenesis Group, Centre for Genomics and Personalised Health, Genomics Research Centre, School of Biomedical Sciences, Queensland University of Technology (QUT), 60 Musk Ave., Kelvin Grove, Queensland 4059, Australia.
² ARC Training Centre for Cell and Tissue Engineering Technologies, Queensland University of Technology (QUT), Australia.

Abstract

To obtain this dataset, two human HER2-positive breast cancer cell lines (SKBR3 and MDA-MB-453 cell lines) were cultured in basal growth media to 80% confluence. Cells were passaged and total RNA extracted, RNA converted to cDNA and diluted to a working concentration of 40 ng/µL. Gene expression panels of cancer markers including Fibroblast growth factors (FGF), FGF receptors (FGFRs), cyclin-dependent kinases, cytokeratins, and WNT pathway components were then examined using Q-PCR. Gene expression was normalised against the expression of the endogenous gene 18S. This article describes the data used in the research article "Syndecan-4 regulates the HER2-positive breast cancer cell proliferation cells via CK19/AKT signaling" [1]. The data presented demonstrates the range of gene expression profiles of these cells and aims to provide more detail of all gene expression changes observed in these cell lines.

Keywords: Cell proliferation; Cytokeratin 19; Heparan sulfate proteoglycans; Heparin; Human breast cancers; MDA-MB-453; SKBR3.