The cellular model for Alzheimer's disease research: PC12 cells

Danni Xie; Ting Deng; Zhenwei Zhai; Tao Sun; Ying Xu

doi:10.3389/fnmol.2022.1016559

The cellular model for Alzheimer's disease research: PC12 cells

Front Mol Neurosci. 2023 Jan 4:15:1016559. doi: 10.3389/fnmol.2022.1016559. eCollection 2022.

Authors

Danni Xie¹, Ting Deng¹, Zhenwei Zhai², Tao Sun^{1

2}, Ying Xu³

Affiliations

¹ State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
² School of Medical Information Engineering, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
³ TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Abstract

Alzheimer's disease (AD) is a common age-related neurodegenerative disease characterized by progressive cognitive decline and irreversible memory impairment. Currently, several studies have failed to fully elucidate AD's cellular and molecular mechanisms. For this purpose, research on related cellular models may propose potential predictive models for the drug development of AD. Therefore, many cells characterized by neuronal properties are widely used to mimic the pathological process of AD, such as PC12, SH-SY5Y, and N2a, especially the PC12 pheochromocytoma cell line. Thus, this review covers the most systematic essay that used PC12 cells to study AD. We depict the cellular source, culture condition, differentiation methods, transfection methods, drugs inducing AD, general approaches (evaluation methods and metrics), and in vitro cellular models used in parallel with PC12 cells.

Keywords: Alzheimer's disease; PC12 cells; culture condition; differentiation methods; drugs inducing AD; in vitro cellular models used in parallel with PC12 cells; transfection methods.

Publication types

Systematic Review