Beneficial properties of Drimia numidica leaf methanolic extract against the cytogenotoxic effects of mitomycin C on human lymphocytes

Margarita Dormousoglou; Vasiliki Boti; Dimitra Hela; Dimitris Vlastos; Maria Antonopoulou; Christos Chondrogiannis; Yiola Petropoulou; Stefanos Dailianis

doi:10.1016/j.fct.2023.113626

Beneficial properties of Drimia numidica leaf methanolic extract against the cytogenotoxic effects of mitomycin C on human lymphocytes

Food Chem Toxicol. 2023 Mar:173:113626. doi: 10.1016/j.fct.2023.113626. Epub 2023 Jan 20.

Authors

Margarita Dormousoglou¹, Vasiliki Boti², Dimitra Hela³, Dimitris Vlastos⁴, Maria Antonopoulou⁵, Christos Chondrogiannis⁴, Yiola Petropoulou⁴, Stefanos Dailianis⁶

Affiliations

¹ Department of Biology, School of Natural Sciences, University of Patras, GR-26500, Rio, Patras, Greece; Department of Sustainable Agriculture, University of Patras, GR-30100, Agrinio, Greece.
² Department of Chemistry, University of Ioannina, GR-45110, Ioannina, Greece; Institute of Environment and Sustainable Development, University Research Center of Ioannina (URCI), Ioannina, GR-45110, Greece; Unit of Environmental, Organic and Biochemical High-resolution Analysis-Orbitrap-LC-MS, University of Ioannina, Ioannina, GR-45110, Greece.
³ Department of Chemistry, University of Ioannina, GR-45110, Ioannina, Greece; Institute of Environment and Sustainable Development, University Research Center of Ioannina (URCI), Ioannina, GR-45110, Greece.
⁴ Department of Biology, School of Natural Sciences, University of Patras, GR-26500, Rio, Patras, Greece.
⁵ Department of Sustainable Agriculture, University of Patras, GR-30100, Agrinio, Greece.
⁶ Department of Biology, School of Natural Sciences, University of Patras, GR-26500, Rio, Patras, Greece. Electronic address: sdailianis@upatras.gr.

PMID: 36682415
DOI: 10.1016/j.fct.2023.113626

Abstract

This study investigated the phytochemical profile of Drimia numidica leaf methanolic extract, as well as its cyto-genotoxic and cyto/genoprotective potential against mitomycin C (MMC) mediated effects on healthy human lymphocytes. Photosynthetic pigments, trace elements, and secondary metabolites were estimated and/or identified in methanolic extract of mature leaves, and the latter was further used for assessing its in vitro biological effects on MMC-free and/or MMC-treated human lymphocytes (at low, non-toxic concentrations of 0.001 and 0.01% v/v). The results showed that D. numidica leaf methanolic extract, being rich in carotenoids, phenolics, flavonoids, organic acids and bufadienolides, could be protective against MMC mediated cyto/genotoxic potential in healthy human lymphocytes. Biomolecules possessing antioxidant and antitumor potential, such as beta-carotene and lutein among others, chlorogenic acid, caffeic acid and their derivatives, minerals such as Si, as well as apigenin- and luteolin-derived glycosides, either individual or in a mixture, could be beneficial rather than harmful, at least at the extract concentrations tested. Although further in vitro and in vivo studies are still needed for elucidating the beneficial (individual and/or additive/synergistic) role of those compounds, the results of the present study are quite promising, thus encouraging new challenges for the appropriate utilization of D. numidica leaf extract.

Keywords: CBMN assay; Cyto/genotoxicity; Drimia numidica; Human lymphocytes; Leaf methanolic extract; Secondary metabolites.

MeSH terms

DNA Damage
Drimia* / chemistry
Humans
Lymphocytes
Mitomycin* / toxicity
Plant Extracts / pharmacology
Plant Leaves

Substances

Mitomycin
Plant Extracts