Novel evaluation indicators of MOG35∼55 induced experimental autoimmune encephalomyelitis in C57BL/6J mice

Chun Wang; Jie Lv; Qiaoling Zhu; Wei Zhuang; Ling Xie; Guangyu Liu; Kaidireya Saimaier; Changjie Shi; Qiuhong Hua; Rui Yue; Changsheng Du

doi:10.1016/j.imbio.2023.152341

Novel evaluation indicators of MOG_35∼55 induced experimental autoimmune encephalomyelitis in C57BL/6J mice

Immunobiology. 2023 Mar;228(2):152341. doi: 10.1016/j.imbio.2023.152341. Epub 2023 Jan 18.

Authors

Chun Wang¹, Jie Lv¹, Qiaoling Zhu², Wei Zhuang³, Ling Xie¹, Guangyu Liu¹, Kaidireya Saimaier¹, Changjie Shi¹, Qiuhong Hua¹, Rui Yue², Changsheng Du⁴

Affiliations

¹ Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Orthopaedic Department of Tongji Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
² Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
³ Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Orthopaedic Department of Tongji Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
⁴ Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Orthopaedic Department of Tongji Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China. Electronic address: ducs2015@163.com.

PMID: 36680977
DOI: 10.1016/j.imbio.2023.152341

Abstract

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS), characterized by demyelinating neuropathy. Despite a long period of research on the immune mechanisms involved in CNS diseases, the etiology of MS remains unknown. MS may present with different clinical and pathological manifestations due to the involvement of different pathogenic processes, including balance and mobility disorders, psychiatric abnormalities, and intestinal dysfunction. We used an animal model of MS, experimental autoimmune encephalomyelitis (EAE), to assess clinical symptoms of MS with the aim of creating new indicators for the assessment of EAE. Our results show that EAE mice develop severe bone loss, anxiety-like moods, and intestinal inflammation in addition to clinical phenomena such as inflammatory infiltration and demyelination of the spinal cord. Our new indicators aim to provide a more comprehensive assessment of MS to avoid the pitfalls of a single intervention and also to provide a more systematic assessment of the effectiveness of drugs used to treat MS.

Keywords: Anxiety-like behavior; Experimental autoimmune encephalomyelitis; Intestinal inflammation; Multiple sclerosis; Osteoporosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Central Nervous System
Encephalomyelitis, Autoimmune, Experimental*
Mice
Mice, Inbred C57BL
Multiple Sclerosis*
Spinal Cord