Myogenesis and Analysis of Antimicrobial Potential of Silver Nanoparticles (AgNPs) against Pathogenic Bacteria

Palwasha Hayat; Ibrar Khan; Aneela Rehman; Tayyaba Jamil; Azam Hayat; Mujaddad Ur Rehman; Najeeb Ullah; Abid Sarwar; Amnah A Alharbi; Anas S Dablool; Zubaida Daudzai; Abdulhakeem S Alamri; Majid Alhomrani; Tariq Aziz

doi:10.3390/molecules28020637

Myogenesis and Analysis of Antimicrobial Potential of Silver Nanoparticles (AgNPs) against Pathogenic Bacteria

Molecules. 2023 Jan 7;28(2):637. doi: 10.3390/molecules28020637.

Authors

Affiliations

¹ Department of Microbiology, Abbottabad University of Science & Technology, Havelian 22010, Pakistan.
² Food and Biotechnology Research Center, PCSIR Labs Complex Lahore, Lahore 54600, Pakistan.
³ Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia.
⁴ Department of Public Health, Health Sciences College Al-Leith, Umm Al-Qura University, Makkah 24382, Saudi Arabia.
⁵ Department of Bioresource and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.
⁶ Department of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
⁷ School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

Abstract

The widespread and indiscriminate use of broad-spectrum antibiotics leads to microbial resistance, which causes major problems in the treatment of infectious diseases. However, advances in nanotechnology have opened up new domains for the synthesis and use of nanoparticles against multidrug-resistant pathogens. The traditional approaches for nanoparticle synthesis are not only expensive, laborious, and hazardous but also have various limitations. Therefore, new biological approaches are being designed to synthesize economical and environmentally friendly nanoparticles with enhanced antimicrobial activity. The current study focuses on the isolation, identification, and screening of metallotolerant fungal strains for the production of silver nanoparticles, using antimicrobial activity analysis and the characterization of biologically synthesized silver nanoparticles by X-ray diffraction (XRD) spectroscopy, energy-dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM). In total, 11 fungal isolates were isolated and screened for the synthesis of AgNPs, while the Penicillium notatum (K1) strain was found to be the most potent, demonstrating biosynthetic ability. The biologically synthesized silver nanoparticles showed excellent antibacterial activity against the bacteria Escherichia coli (ATCC10536), Bacillus subtilis, Staphylococcus aureus (ATCC9144), Pseudomonas aeruginosa (ATCC10145), Enterococcus faecalis, and Listeria innocua (ATCC13932). Furthermore, three major diffraction peaks in the XRD characterization, located at the 2θ values of 28.4, 34.8, 38.2, 44, 64, and 77°, confirmed the presence of AgNPs, while elemental composition analysis via EDX and spherical surface topology with a scanning electron microscope indicated that its pure crystalline nature was entirely composed of silver. Thus, the current study indicates the enhanced antibacterial capability of mycologically synthesized AgNPs, which could be used to counter multidrug-resistant pathogens.

Keywords: EDX; MDR; Penicillium notatum; SEM1; XDR; myogenesis; silver nanoparticles.

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Infective Agents* / chemistry
Anti-Infective Agents* / pharmacology
Bacteria
Metal Nanoparticles* / chemistry
Microbial Sensitivity Tests
Muscle Development
Plant Extracts / chemistry
Silver / chemistry
Silver / pharmacology
Spectrometry, X-Ray Emission
Spectroscopy, Fourier Transform Infrared

Substances

Silver
Anti-Infective Agents
Anti-Bacterial Agents
Plant Extracts

Grants and funding

The authors extend their appreciation to the Deputyship of Research and Innovation, Ministry of Education in Saudi Arabia for funding this research work through the project number TUIF-23A-9-9.