A novel nitrogen-fixing bacterium, Propionivibrio soli sp. nov. isolated from paddy soil

Abstract

A facultative anaerobic nitrogen-fixing bacterium, designated SG131^T, was isolated from paddy soil. Strain SG131^T showed high 16S rRNA gene sequence similarities with type strains Propionivibrio limicola DSM 6832^T (96.9%), Propionivibrio pelophilus asp 66^T (96.0%) and Propionivibrio dicarboxylicus DSM 5885^T (95.7%). The phylogenetic trees (based on 16S rRNA gene sequences and 120 conserved genes from genomes, respectively) indicated that strain SG131^T clustered with members of the genus Propionivibrio. Growth of strain SG131^T was observed at 25-40 °C, pH 5.5-10.5 and 0-0.5% (w/v) NaCl. The quinone was Q-7, and the main fatty acids were C_16:1 ω6c and/or C_16:1 ω7c (25.9%), C_16:0 (23.3%), C_17:0-cyclo (11.7%), C_12:0 (6.0%) and C_17:0 (5.9%). The genomic DNA G + C content of strain SG131^T was 60.3%. The average nucleotide identity (ANI) values between strain SG131^T and its most closely related species P. limicola DSM 6832^T, P. pelophilus DSM 12018^T and P. dicarboxylicus DSM 5885^T were 74.4%, 74.9% and 75.6%, respectively. The digital DNA-DNA hybridization (dDDH) values between strain SG131^T and its most closely related species P. limicola DSM 6832^T, P. pelophilus DSM 12018^T and P. dicarboxylicus DSM 5885^T were 19.9%, 20.6% and 20.5%, respectively. All these values were lower than the recommended species delineation thresholds of ANI (95-96%) and dDDH (70%). Strain SG131^T possessed core genes (nifHDK) of nitrogen fixation and was confirmed its nitrogen-fixing ability by the ARA method. According to the above-described analysis, strain SG131^T represents a novel species of the genus Propionivibrio, for which the name Propionivibrio soli sp. nov. is proposed. The type strain is SG131^T (= GDMCC 1.3313^T = JCM 35595^T).

Keywords: Nitrogen fixation; Paddy soil; Polyphasic taxonomy; Propionivibrio soli sp. nov..