Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells

Jeonghun Baek; Chun Wai Mai; Wei Meng Lim; Lai Chun Wong

doi:10.18240/ijo.2023.01.06

Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells

Int J Ophthalmol. 2023 Jan 18;16(1):40-46. doi: 10.18240/ijo.2023.01.06. eCollection 2023.

Authors

Jeonghun Baek¹, Chun Wai Mai², Wei Meng Lim³, Lai Chun Wong²

Affiliations

¹ School of Medicine, International Medical University, Kuala Lumpur 57000, Malaysia.
² Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University, Kuala Lumpur 57000, Malaysia.
³ Department of Pharmaceutical Technology, School of Pharmacy, International Medical University, Kuala Lumpur 57000, Malaysia.

Abstract

Aim: To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial (ARPE)-19 cells in vitro.

Methods: ARPE-19 cells were cultured in Dulbecco's Modified Eagle Medium-F-12 supplemented with 10% foetal bovine serum and 1% penicillin-streptomycin in a humidified 5% CO₂ incubator maintained at 37°C. Cells were treated with 247 µmol/L lutein, 49 µmol/L zeaxanthin and 1% (v/v) of either coconut oil, corn oil, peanut oil, olive oil, sunflower oil, soybean oil, castor oil, or linseed oil for 48h. Lutein and zeaxanthin concentration in the cells were quantified by high performance liquid chromatography.

Results: Among the oils tested, the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed oil.

Conclusion: ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils.

Keywords: adult retinal pigment epithelial-19 cells; lutein; vegetable oil; zeaxanthin.

International Journal of Ophthalmology Press.