Detecting STR profiles from degrading menstrual blood samples and their use as possible evidence in forensic investigations

Tahani Mohamed Ibrahim Al-Hazani; Wedad Saeed Al-Qahtani; Tahani Saeed Abboosh; Fatmah Ahmed Safhi; Dalal S Alshaya; Areej Saud Jalal; Salha M Al-Shamrani; Nouf Abdallah Al-Ghamdi; Amani Mohammed Alotaibi; Mashael Alhumaidi Alotaibi; Hanan S Alghamdi; Hayat Ali Alafari; Tahani Ahmad ALMatrafi; Eman Alshehri

doi:10.1016/j.forsciint.2023.111562

Detecting STR profiles from degrading menstrual blood samples and their use as possible evidence in forensic investigations

Forensic Sci Int. 2023 Feb:343:111562. doi: 10.1016/j.forsciint.2023.111562. Epub 2023 Jan 7.

Authors

Affiliations

¹ Department of Biology, College of Sciences and Humanities, Prince Sattam Bin Abdulaziz University, P.O. Box: 83, Al-Kharj 11940, Saudi Arabia. Electronic address: tahanihazani@gmail.com.
² Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, P.O. Box 6830, Riyadh 11452, Saudi Arabia. Electronic address: wed-mw@hotmail.com.
³ Ministry of Interior, Public Security, Forensic Evidence Laboratories, Criminal Examinations, Riyadh, Saudi Arabia. Electronic address: tahani.abboosh1986@gmail.com.
⁴ Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: faalsafhi@pnu.edu.sa.
⁵ Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: dsalshaya@pnu.edu.sa.
⁶ Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: asjalal@pnu.edu.sa.
⁷ University of Jeddah, College of Science, Department of Biology, P.O. Box 13151, Jeddah 21493, Saudi Arabia. Electronic address: smalshmrane@uj.edu.sa.
⁸ Department of Zoology, College of Science, King Saud University, Saudi Arabia. Electronic address: nam.1408@hotmail.com.
⁹ King Saud Medical City, Riyadh, Saudi Arabia. Electronic address: dr.dooode@gmail.com.
¹⁰ Department of Biology, College of Science, Jouf University, P.O. Box 2014, Sakaka, Saudi Arabia. Electronic address: maotaibi@ju.edu.sa.
¹¹ Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. Electronic address: H_alghamdi_@hotmail.com.
¹² Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: haalafari@pnu.edu.sa.
¹³ Anatomy Department, College of Medicine, King Saud University, Saudi Arabia. Electronic address: talmatrafi@ksu.edu.sa.
¹⁴ Department of Zoology, College of Science, King Saud University, Saudi Arabia. Electronic address: ealshehri@ksu.edu.sa.

PMID: 36657183
DOI: 10.1016/j.forsciint.2023.111562

Abstract

This research explores DNA consistency and attempts to detect STR profiles from the degrading menstrual blood samples (MBS) as reliable forensic evidence. Peripheral (PBS) and MBS of 30 healthy fertile females were taken on the menstrual cycle's second day. They were obtained at different time periods (0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 24, and 48 h) at 25 °C. DNA evaluation was fulfilled to analyze DNA profiles. A considerable elevation in the median concentrations of DNA between 0 and 14-h intervals were documented, whereas decreased extents were registered between 16 and 48 h. Moreover, complete STR profiles (24/24) for DNA were discovered in all the intervals (0, 2, and 48 h). Periods of 0-8 h demonstrated the maximum extents of DNA materials. Full STR were discovered in all the intervals (0, 2, and 48 h). Eventually, MBS can be utilized as forensic evidence.

Keywords: Criminal evidence; Degrading menstrual blood samples; Female victims; Forensic investigations; STR profile.

MeSH terms

DNA / genetics
DNA Fingerprinting*
Female
Humans
Microsatellite Repeats*

Substances

DNA