Infrared Spectroscopy for Structure Analysis of Protein Inclusion Bodies

Andreas Schwaighofer; Bernhard Lendl

doi:10.1007/978-1-0716-2930-7_15

Infrared Spectroscopy for Structure Analysis of Protein Inclusion Bodies

Methods Mol Biol. 2023:2617:209-223. doi: 10.1007/978-1-0716-2930-7_15.

Authors

Andreas Schwaighofer¹, Bernhard Lendl²

Affiliations

¹ Research Group Process Analytics, Institute of Chemical Technologies and Analytics, TU Wien, Vienna, Austria. andreas.schwaighofer@tuwien.ac.at.
² Research Group Process Analytics, Institute of Chemical Technologies and Analytics, TU Wien, Vienna, Austria.

PMID: 36656527
DOI: 10.1007/978-1-0716-2930-7_15

Abstract

Infrared (IR) spectroscopy is a widely used technique for evaluation of protein secondary structure. In this chapter, we focus on the application of this analytical technique for analysis of inclusion bodies. After a general introduction to protein analysis by IR spectroscopy, different approaches for spectra acquisition, data processing, and secondary structure evaluation are presented.

Keywords: Infrared spectroscopy; Protein structure; Secondary structure analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Inclusion Bodies* / metabolism
Protein Structure, Secondary
Proteins* / chemistry
Spectrophotometry, Infrared
Spectroscopy, Fourier Transform Infrared / methods

Substances

Proteins