Single-cell atlas of domestic pig cerebral cortex and hypothalamus

Jiacheng Zhu; Fang Chen; Lihua Luo; Weiying Wu; Jinxia Dai; Jixing Zhong; Xiumei Lin; Chaochao Chai; Peiwen Ding; Langchao Liang; Shiyou Wang; Xiangning Ding; Yin Chen; Haoyu Wang; Jiaying Qiu; Feiyue Wang; Chengcheng Sun; Yuying Zeng; Jian Fang; Xiaosen Jiang; Ping Liu; Gen Tang; Xin Qiu; Xingliang Zhang; Yetian Ruan; Sanjie Jiang; Jiankang Li; Shida Zhu; Xun Xu; Fang Li; Zhongmin Liu; Gang Cao; Dongsheng Chen

doi:10.1016/j.scib.2021.04.002

Single-cell atlas of domestic pig cerebral cortex and hypothalamus

Sci Bull (Beijing). 2021 Jul 30;66(14):1448-1461. doi: 10.1016/j.scib.2021.04.002. Epub 2021 Apr 6.

Authors

Jiacheng Zhu¹, Fang Chen², Lihua Luo¹, Weiying Wu³, Jinxia Dai⁴, Jixing Zhong⁵, Xiumei Lin¹, Chaochao Chai¹, Peiwen Ding¹, Langchao Liang¹, Shiyou Wang¹, Xiangning Ding¹, Yin Chen¹, Haoyu Wang¹, Jiaying Qiu¹, Feiyue Wang⁶, Chengcheng Sun⁷, Yuying Zeng⁸, Jian Fang⁶, Xiaosen Jiang¹, Ping Liu², Gen Tang⁹, Xin Qiu⁹, Xingliang Zhang⁹, Yetian Ruan¹⁰, Sanjie Jiang⁶, Jiankang Li⁶, Shida Zhu⁶, Xun Xu⁶, Fang Li¹⁰, Zhongmin Liu¹⁰, Gang Cao¹¹, Dongsheng Chen¹²

Affiliations

¹ College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI-Shenzhen, Shenzhen 518083, China.
² BGI-Shenzhen, Shenzhen 518083, China; MGI, BGI-Shenzhen, Shenzhen 518083, China.
³ BGI-Shenzhen, Shenzhen 518083, China; Department of Neurobiology, NHC and CAMS Key Laboratory of Medical Neurobiology, School of Brain Science and Brian Medicine, and the MOE Frontier Science Center for Brain Research and Brain-Machine Integration, Zhejiang University School of Medicine, Hangzhou 310031, China.
⁴ State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
⁵ School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.
⁶ BGI-Shenzhen, Shenzhen 518083, China.
⁷ College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI-Shenzhen, Shenzhen 518083, China; School of Basic Medicine, Qingdao University, Qingdao 266071, China.
⁸ College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI-Shenzhen, Shenzhen 518083, China; College of Life Science, South China Agricultural University, Guangzhou 510642, China.
⁹ Shenzhen Children's Hospital, Shenzhen 518083, China.
¹⁰ Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
¹¹ State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; College of Biomedicine and Health, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: gcao@mail.hzau.edu.cn.
¹² BGI-Shenzhen, Shenzhen 518083, China. Electronic address: chendongsheng@genomics.cn.

PMID: 36654371
DOI: 10.1016/j.scib.2021.04.002

Abstract

The brain of the domestic pig (Sus scrofa domesticus) has drawn considerable attention due to its high similarities to that of humans. However, the cellular compositions of the pig brain (PB) remain elusive. Here we investigated the single-nucleus transcriptomic profiles of five regions of the PB (frontal lobe, parietal lobe, temporal lobe, occipital lobe, and hypothalamus) and identified 21 cell subpopulations. The cross-species comparison of mouse and pig hypothalamus revealed the shared and specific gene expression patterns at the single-cell resolution. Furthermore, we identified cell types and molecular pathways closely associated with neurological disorders, bridging the gap between gene mutations and pathogenesis. We reported, to our knowledge, the first single-cell atlas of domestic pig cerebral cortex and hypothalamus combined with a comprehensive analysis across species, providing extensive resources for future research regarding neural science, evolutionary developmental biology, and regenerative medicine.

Keywords: Brain cell atlas; Domestic pig; Neural science; Single-cell transcriptome.