An In Vivo Model to Study Cell Migration in XYZ-T Dimension Followed by Whole-Mount Re-evaluation

Ann L B Seynhaeve; Timo L M Ten Hagen

doi:10.1007/978-1-0716-2887-4_19

An In Vivo Model to Study Cell Migration in XYZ-T Dimension Followed by Whole-Mount Re-evaluation

Methods Mol Biol. 2023:2608:325-341. doi: 10.1007/978-1-0716-2887-4_19.

Authors

Ann L B Seynhaeve¹, Timo L M Ten Hagen²

Affiliations

¹ Laboratory Experimental Oncology, Department of Pathology, Erasmus MC, Rotterdam, the Netherlands. a.seynhaeve@erasmusmc.nl.
² Laboratory Experimental Oncology, Department of Pathology, Erasmus MC, Rotterdam, the Netherlands.

PMID: 36653716
DOI: 10.1007/978-1-0716-2887-4_19

Abstract

Cell migration is a very dynamic process involving several chemical as well as biological interactions with other cells and the environment. Several models exist to study cell migration ranging from simple 2D in vitro cultures to more demanding 3D multicellular assays, to complex evaluation in animals. High-resolution 4D (XYZ, spatial + T, time dimension) intravital imaging using transgenic animals with a fluorescent label in cells of interest is a powerful tool to study cell migration in the correct environment. Here we describe an advanced dorsal skinfold chamber model to study endothelial cell and pericyte migration and association.

Keywords: Cell migration; Endothelial cells; Intravital microscopy; Pericytes; Whole-mount staining.

MeSH terms

Animals
Cell Movement
Intravital Microscopy* / methods
Pericytes*