Nuclear SPHK2/S1P induces oxidative stress and NLRP3 inflammasome activation via promoting p53 acetylation in lipopolysaccharide-induced acute lung injury

Linjing Gong; Yue Shen; Sijiao Wang; Xinyuan Wang; Haiying Ji; Xu Wu; Lijuan Hu; Lei Zhu

doi:10.1038/s41420-023-01320-5

Nuclear SPHK2/S1P induces oxidative stress and NLRP3 inflammasome activation via promoting p53 acetylation in lipopolysaccharide-induced acute lung injury

Cell Death Discov. 2023 Jan 18;9(1):12. doi: 10.1038/s41420-023-01320-5.

Authors

Linjing Gong^{1

2}, Yue Shen², Sijiao Wang², Xinyuan Wang³, Haiying Ji², Xu Wu², Lijuan Hu², Lei Zhu^{4

5}

Affiliations

¹ Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, No 37 Guoxue Alley, 610041, Chengdu, Sichuan, China.
² Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, 200032, Shanghai, China.
³ Department of Orthopaedics, West China Hospital, Sichuan University, No 37 Guoxue Alley, 610041, Chengdu, Sichuan, China.
⁴ Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, 200032, Shanghai, China. tfzhu@126.com.
⁵ Department of Respiratory and Critical Care Medicine, Beijing Tsinghua Changgung Hospital affiliated to Tsinghua University, 102218, Beijing, China. tfzhu@126.com.

Abstract

A bulk of evidence identified that macrophages, including resident alveolar macrophages and recruited macrophages from the blood, played an important role in the pathogenesis of acute respiratory distress syndrome (ARDS). However, the molecular mechanisms of macrophages-induced acute lung injury (ALI) by facilitating oxidative stress and inflammatory responses remain unclear. Herein, we noticed that the levels of mitochondrial reactive oxygen species (mtROS), SPHK2 and activated NLRP3 inflammasome were higher in peripheral blood mononuclear cells (PBMCs) of ARDS patients than that in healthy volunteers. Similar observations were recapitulated in LPS-treated RAW264.7 and THP-1 cells. After exposure to LPS, the SPHK2 enzymatic activity, NLRP3 inflammasome activation and mtROS were significantly upregulated in macrophages. Moreover, knockdown SPHK2 via shRNA or inhibition SPHK2 could prominently decrease LPS-induced M1 macrophage polarization, oxidative stress and NLRP3 inflammasome activation. Further study indicated that upregulated SPHK2 could increase nuclear sphingosine-1-phosphate (S1P) levels and then restrict the enzyme activity of HDACs to facilitate p53 acetylation. Acetylation of p53 reinforced its binding to the specific region of the NLRP3 promoter and drove expression of NLRP3. In the in vivo experiments, it was also observed that treating with Opaganib (ABC294640), a specific SPHK2 inhibitor, could observably alleviate LPS-induced ALI, evidencing by lowered infiltration of inflammatory cells, increased M2 macrophages polarization and reduced oxidative damage in lung tissues. Besides, SPHK2 inhibition can also decrease the accumulation of acetylated p53 protein and the activation of NLRP3 inflammasome. Taken together, our results demonstrated for the first time that nuclear S1P can regulate the acetylation levels of non-histone protein through affecting HDACs enzyme activities, linking them to oxidative stress and inflammation in response to environmental signals. These data provide a theoretical basis that SPHK2 may be an effective therapeutic target of ARDS.