Sparassis latifolia polysaccharides inhibit colon cancer in mice by modulating gut microbiota and metabolism

Xin Wei; Feier Cheng; Jingyu Liu; Yanfen Cheng; Shaojun Yun; Junlong Meng; Mingchang Chang; Xueran Geng; Lijing Xu; Jinling Cao; Cuiping Feng

doi:10.1016/j.ijbiomac.2023.123299

Sparassis latifolia polysaccharides inhibit colon cancer in mice by modulating gut microbiota and metabolism

Int J Biol Macromol. 2023 Mar 31:232:123299. doi: 10.1016/j.ijbiomac.2023.123299. Epub 2023 Jan 14.

Authors

Xin Wei¹, Feier Cheng¹, Jingyu Liu², Yanfen Cheng³, Shaojun Yun³, Junlong Meng¹, Mingchang Chang¹, Xueran Geng³, Lijing Xu³, Jinling Cao⁴, Cuiping Feng⁵

Affiliations

¹ College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
² College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Shanxi Key Laboratory of Edible Fungi for Loess Plateau, Taigu, Shanxi 030801, China; Collaborative Innovation Center of Quality and Efficiency of Loess Plateau Edible Fungi, Taigu, Shanxi 030801, China.
³ College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Shanxi Key Laboratory of Edible Fungi for Loess Plateau, Taigu, Shanxi 030801, China.
⁴ College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Shanxi Key Laboratory of Edible Fungi for Loess Plateau, Taigu, Shanxi 030801, China. Electronic address: caojinling7928@163.com.
⁵ College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Shanxi Key Laboratory of Edible Fungi for Loess Plateau, Taigu, Shanxi 030801, China. Electronic address: ndfcp@163.com.

PMID: 36649865
DOI: 10.1016/j.ijbiomac.2023.123299

Abstract

Sparassis latifolia polysaccharides (SLPs) can regulate inflammatory cytokines. However, little is known about the regulation mechanism of SLPs on colon cancer. In this study, we investigated the mechanism of SLPs on metabolism in mice with colon cancer. The results showed that SLPs could improve the colon morphology and physiological indices, and inhibit the infiltration of immune cells in colon. Moreover, it could improve metabolism disorder of colon cancer via reducing the levels of TNF-α, IL-6, NF-κB, COX-2 and IL-1β mRNA or protein, increasing IκB mRNA or protein expression. In addition, it could comprehensively regulate the colon cancer related metabolism by changing the abundance of key intestinal flora and 35 metabolites including phosphatidylcholine, tryptophan and tetrahydrobiopterin. Some biomarkers associated with colon cancer metabolism were related significantly with the abundance of specific intestinal flora. These findings indicate that SLPs can attenuate metabolism disorder of colon cancer by modulating gut microbiota and metabolites.

Keywords: Colon cancer; Intestinal microorganisms; Metabolites; Sparassis latifolia polysaccharides.

MeSH terms

Animals
Colon
Colonic Neoplasms* / drug therapy
Colonic Neoplasms* / metabolism
Dextran Sulfate
Disease Models, Animal
Gastrointestinal Microbiome*
Mice
Mice, Inbred C57BL
Polysaccharides / metabolism
Polysaccharides / pharmacology
RNA, Messenger / metabolism

Substances

Polysaccharides
RNA, Messenger
Dextran Sulfate

Supplementary concepts

Sparassis latifolia