Transcriptome and metabolome response of eggplant against Ralstonia solanacearum infection

Xi Ou Xiao; Wenqiu Lin; Enyou Feng; Xiongchang Ou

doi:10.7717/peerj.14658

Transcriptome and metabolome response of eggplant against Ralstonia solanacearum infection

PeerJ. 2023 Jan 11:11:e14658. doi: 10.7717/peerj.14658. eCollection 2023.

Authors

Xi Ou Xiao^{1

2

3}, Wenqiu Lin^{1

2

3}, Enyou Feng⁴, Xiongchang Ou^{1

2

3}

Affiliations

¹ South Subtropical Crop Research Institute Chinese Academy of Tropical Agricultural Sciences (CATAS), Zhanjiang, Guangdong, China.
² Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, Guagndong, China.
³ Zhanjiang City Key Laboratory for Tropical Crops Genetic Improvement, Zhanjiang, Guangdong, China.
⁴ Zhanjiang Academy of Agricultural Sciences, Zhanjiang, Guangdong, China.

Abstract

Bacterial wilt is a soil-borne disease that represents ubiquitous threat to Solanaceae crops. The whole-root transcriptomes and metabolomes of bacterial wilt-resistant eggplant were studied to understand the response of eggplant to bacterial wilt. A total of 2,896 differentially expressed genes and 63 differences in metabolites were identified after inoculation with Ralstonia solanacearum. Further analysis showed that the biosynthesis pathways for phytohormones, phenylpropanoids, and flavonoids were altered in eggplant after inoculation with R. solanacearum. The results of metabolomes also showed that phytohormones played a key role in eggplant response to bacterial wilt. Integrated analyses of the transcriptomic and metabolic datasets indicated that jasmonic acid (JA) content and gene involved in the JA signaling pathway increased in response to bacterial wilt. These findings remarkably improve our understanding of the mechanisms of induced defense response in eggplant and will provide insights intothe development of disease-resistant varieties of eggplant.

Keywords: Bacterial wilt; Eggplant; JA signaling pathway; Metabolomes; Transcriptomes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Metabolome / genetics
Plant Growth Regulators / metabolism
Ralstonia solanacearum* / genetics
Solanum melongena* / genetics
Transcriptome / genetics

Substances

Plant Growth Regulators

Grants and funding

This research was supported by the National Natural Science Foundation of China (No. 31872117), the Central Public-interest Scientific Institution Basal Research Fund (No. 1630062022003), the Central Public-interest Scientific Institution Basal Research Program for Scientific Research Innovation Team (No. 1630062017014), and the National Natural Science Foundation of Hainan Province (No. 321RC632). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.