Characterization of CD3γ/δ gene and its immune response in Qihe crucian carp Carassius auratus after challenged by Aeromonas veronii and Poly(I:C)

Hairong Tang; Lei Zhu; Xianliang Zhao; Xinyu Jiang; Jie Zhang; Chao Pei; Li Li; Xianghui Kong

doi:10.1016/j.fsi.2023.108550

Characterization of CD3γ/δ gene and its immune response in Qihe crucian carp Carassius auratus after challenged by Aeromonas veronii and Poly(I:C)

Fish Shellfish Immunol. 2023 Feb:133:108550. doi: 10.1016/j.fsi.2023.108550. Epub 2023 Jan 13.

Authors

Hairong Tang¹, Lei Zhu¹, Xianliang Zhao², Xinyu Jiang², Jie Zhang², Chao Pei², Li Li², Xianghui Kong³

Affiliations

¹ College of Life Science, Henan Normal University, Henan province, PR China; Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Henan province, PR China.
² Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Henan province, PR China.
³ College of Life Science, Henan Normal University, Henan province, PR China; Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Henan province, PR China. Electronic address: xhkong@htu.cn.

PMID: 36646341
DOI: 10.1016/j.fsi.2023.108550

Abstract

CD3γ/δ found in non-mammalian vertebrates is a CD3 homolog with structural characteristics similar to both mammalian CD3γ and CD3δ, and plays important roles in T cell recognization and immune response in fish. In this study, the full-length of CD3γ/δ from Qihe crucian carp (named CaCD3γ/δ) was cloned and characterized, then the expression response profiles and potential immune functions was explored after Aeromonas veronii and Poly(I:C) challenge. The results showed that the full-length of CaCD3γ/δ was 819 bp including a 5'-UTR of 141 bp, a 3'-UTR of 168 bp, and an ORF of 510 bp encoding a putative 169-aa protein with an estimated MW of 18.71 kD and a theoretical pI of 8.77. The protein sequence of CaCD3γ/δ contained a Leu-Leu and a CXXXC motif in the extracellular domain, and an ITAM and a Leu-Ile motif in the cytoplasm, and a residue of Asn in the transmembrane. CaCD3γ/δ was constitutively expressed in the spleen, liver, gill, and blood of Qihe crucian carp. After the carp were challenged with Poly(I:C) and Aeromonas veronii, the mRNA expression levels of CaCD3γ/δ were significantly changed in the spleen, head kidney, intestine and gill, according to the results of qPCR. However, compared with A. veronii, Poly(I:C) challenge can rapidly induce the CaCD3γ/δ expression levels in head kidney, intestine and spleen, which suggested CaCD3γ/δ may be differentially modulated by different pathogens. Moreover, the results of immunohistochemical analysis showed that the CaCD3γ/δ⁺ secreted cells in the spleen and gills of Qihe crucian were increased after challenged with Poly(I:C), as well as the spleen challenged with A. veronii, but at different levels. Combined with the fact that vascular congestion, necrosis of parenchymal cells, and inflammatory cells including lymphocytes infiltration were also observed in the gill and spleen of Qihe crucian carp treated with A. veronii and Poly(I:C) revealed by pathological analysis, it was predicted that CaCD3γ/δ⁺ T lymphocytes may participated in the immune response against pathogens. This study will contribute to understand the important role of CaCD3γ/δ⁺ T lymphocytes in the immune response of Qihe crucian carp, and provide new insights for the prevention and treatment of the diseases of Qihe crucian carp.

Keywords: CaCD3γ/δ; Carassius auratus; Expression response; Histopathology change.

MeSH terms

Aeromonas veronii / genetics
Animals
Carps* / genetics
Carps* / metabolism
Fish Diseases*
Fish Proteins / chemistry
Goldfish
Immunity, Innate / genetics
Mammals / metabolism
Sequence Alignment

Substances

CD3 antigen, gamma chain
Fish Proteins